Pseudomonas syringae pv. syringae produces two classes of pore-forming lipodepsipeptide phytotoxins that target host plasma membranes. Both syringomycin and syringopeptin are synthesised by modular nonribosomal peptide synthetases. The syringomycin (syr) and syringopeptin (syp) gene clusters are located adjacent to one another on the chromosome and are estimated to be 55 kb and 90 kb in size, respectively. Here we describe the functional organisation of the clusters, along with flanking DNA regions, which altogether cover an approximately 155-kb region of the genome. The predominant feature of the toxin clusters is the occurrence of peptide synthetase genes for syringomycin (i.e., syrB1 and syrE) and syringopeptin (i.e., sypA, sypB, and sypC). Associated with the toxin clusters are genes that are predicted to function in toxin secretion and regulation of biosynthesis genes. Progress in characterising functions of specific genes of the syr-syp cluster dedicated to syringomycin, syringopeptin, or both toxins is summarised.
Characteristic of the syr-syp genomic island of Pseudomonas syringae pv.syringae strain B301D / D. C., Gross; Grgurina, Ingeborg; B. K., SCHOLZ SCHROEDER; S. E., Lu. - STAMPA. - (2003), pp. 137-145. (Intervento presentato al convegno 6th International Conference on Pseudomonas syringae Pathovars and Related Pathogens tenutosi a Maratea (PZ) nel Sept. 15-19, 2002).
Characteristic of the syr-syp genomic island of Pseudomonas syringae pv.syringae strain B301D
GRGURINA, Ingeborg;
2003
Abstract
Pseudomonas syringae pv. syringae produces two classes of pore-forming lipodepsipeptide phytotoxins that target host plasma membranes. Both syringomycin and syringopeptin are synthesised by modular nonribosomal peptide synthetases. The syringomycin (syr) and syringopeptin (syp) gene clusters are located adjacent to one another on the chromosome and are estimated to be 55 kb and 90 kb in size, respectively. Here we describe the functional organisation of the clusters, along with flanking DNA regions, which altogether cover an approximately 155-kb region of the genome. The predominant feature of the toxin clusters is the occurrence of peptide synthetase genes for syringomycin (i.e., syrB1 and syrE) and syringopeptin (i.e., sypA, sypB, and sypC). Associated with the toxin clusters are genes that are predicted to function in toxin secretion and regulation of biosynthesis genes. Progress in characterising functions of specific genes of the syr-syp cluster dedicated to syringomycin, syringopeptin, or both toxins is summarised.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
