A computer modelling study on Candida rugosa lipase/substrate interaction has been carried out. We applied a molecular modeling protocol involving docking experiments followed by extensive conformational analysis of hte compuer-generated complexes. The results of this study highlight possible targets for site-directed mutagenesis. The mutated gene has been expressed and a mutated lipase produced. The activity of hte mew biocatalyst on the substrate of interest has been checked.
Probing the substrate specificity for lipases. Designing a tailor-made enzyme to increase the molecular recognition of 2-arylpropionic esters by Candida rugosa lipase / F., Manetti; F., Corelli; Soro, Simonetta; Palocci, Cleofe; Cernia, Enrico; M., Lotti; M., Botta. - STAMPA. - (1999). (Intervento presentato al convegno Lipases and lipids: structure, function and biotechnological applications tenutosi a Santorini, Greece nel 6-8 maggio 1999).
Probing the substrate specificity for lipases. Designing a tailor-made enzyme to increase the molecular recognition of 2-arylpropionic esters by Candida rugosa lipase
SORO, Simonetta;PALOCCI, Cleofe;CERNIA, Enrico;
1999
Abstract
A computer modelling study on Candida rugosa lipase/substrate interaction has been carried out. We applied a molecular modeling protocol involving docking experiments followed by extensive conformational analysis of hte compuer-generated complexes. The results of this study highlight possible targets for site-directed mutagenesis. The mutated gene has been expressed and a mutated lipase produced. The activity of hte mew biocatalyst on the substrate of interest has been checked.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
