Pectin is one of the main components of primary plant cell wall and its structure is critical for plant growth, intercellular adhesion, fruit texture and interaction with pathogens. Pectin is secreted in a highly methyl-esterified form and subsequently de-esterified by pectin methylesterases (PMEs) localized in the wall. PMEs take part in important physiological processes such as pollen growth and fruit ripening and are involved in the resistance response to fungal and viral pathogens. A mechanism of regulation of PME activity is played by specific proteinaceous inhibitors (PMEI). The structural bases of the interaction between PME from tomato and PMEI from kiwi fruit have been defined by solving the 3D structure of the PME/PMEI complex. We have identified and cloned a fruit-expressed cDNA of tomato with a significant sequence identity with kiwi PMEI. Structural alignment and modelling indicate that the encoded protein has the same fold of kiwi PMEI. Expression and functional characterization of the putative tomato PMEI will be presented .
PECTIN METYLESTERASE INHIBITOR IN TOMATO FRUITS: CLONING AND MOLECULAR CHARACTERIZATION / Reca, Ida Barbara; Laura, Camardella; Rossana, D'Avino; Alfonso, Giovane; Thierry, Giardina; Bellincampi, Daniela. - (2005).
PECTIN METYLESTERASE INHIBITOR IN TOMATO FRUITS: CLONING AND MOLECULAR CHARACTERIZATION
RECA, Ida Barbara;BELLINCAMPI, Daniela
2005
Abstract
Pectin is one of the main components of primary plant cell wall and its structure is critical for plant growth, intercellular adhesion, fruit texture and interaction with pathogens. Pectin is secreted in a highly methyl-esterified form and subsequently de-esterified by pectin methylesterases (PMEs) localized in the wall. PMEs take part in important physiological processes such as pollen growth and fruit ripening and are involved in the resistance response to fungal and viral pathogens. A mechanism of regulation of PME activity is played by specific proteinaceous inhibitors (PMEI). The structural bases of the interaction between PME from tomato and PMEI from kiwi fruit have been defined by solving the 3D structure of the PME/PMEI complex. We have identified and cloned a fruit-expressed cDNA of tomato with a significant sequence identity with kiwi PMEI. Structural alignment and modelling indicate that the encoded protein has the same fold of kiwi PMEI. Expression and functional characterization of the putative tomato PMEI will be presented .I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
