The kinetics of the reaction catalyzed by human erythrocyte Acetylcholinesterase (AChE) is studied in the presence of its inhibitor Tacrine (1,2,3,4-tetrahydro-9-acridinamine), and of two newly synthesized compounds, 6-metoxy-Tacrine and N-eptyl-Tacrine. The proposed kinetic model describes the rate of the enzymatic reaction in terms of competitive and uncompetitive mixed inhibition of the three different inhibitors. The kinetic parameters describing the rate of the reaction are obtained. The competitive and uncompetitive inhibition constants of the different inhibitors are reported and the mixed competitive–uncompetitive inhibition is discussed.
The kinetic inhibition of Acetylcholinesterase from human erytrocytes by tacrine and some tacrine derivatives / F., Porcelli; Delfini, Maurizio; M. R., DEL GIUDICE. - In: BIOORGANIC CHEMISTRY. - ISSN 0045-2068. - 27:3(1999), pp. 197-205. [10.1006/bioo.1998.1121]
The kinetic inhibition of Acetylcholinesterase from human erytrocytes by tacrine and some tacrine derivatives
DELFINI, Maurizio;
1999
Abstract
The kinetics of the reaction catalyzed by human erythrocyte Acetylcholinesterase (AChE) is studied in the presence of its inhibitor Tacrine (1,2,3,4-tetrahydro-9-acridinamine), and of two newly synthesized compounds, 6-metoxy-Tacrine and N-eptyl-Tacrine. The proposed kinetic model describes the rate of the enzymatic reaction in terms of competitive and uncompetitive mixed inhibition of the three different inhibitors. The kinetic parameters describing the rate of the reaction are obtained. The competitive and uncompetitive inhibition constants of the different inhibitors are reported and the mixed competitive–uncompetitive inhibition is discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.