A new type of biosensor based on the coupling of an enzyme to an ion-selective membrane containing a conducting polymer is evaluated. The results obtained with the enzyme field- effect transistor (ENFET) and the ion-selective electrode (ISE) for the determination of creatinine and urea are compared. The presence of the conducting polymer significantly lowers the detection limit for creatinine by one decade to 10−7 and 10−4 M for the ENFET and ISE, respectively. The determination of urea in urine and serum with the ENFET was carried out, and the results correlated well with those obtained by spectrophotometry.
CHARACTERIZATION OF BIOSENSORS BASED ON MEMBRANES CONTAINING A CONDUCTING POLYMER / M., Battilotti; C., Colapicchioni; I., Giannini; F., Porcelli; Campanella, Luigi; M., Cordatore; Mazzei, Franco; Tomassetti, Mauro. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 221:(1989), pp. 157-161. [10.1016/s0003-2670(00)81949-2]
CHARACTERIZATION OF BIOSENSORS BASED ON MEMBRANES CONTAINING A CONDUCTING POLYMER
CAMPANELLA, Luigi;MAZZEI, Franco;TOMASSETTI, Mauro
1989
Abstract
A new type of biosensor based on the coupling of an enzyme to an ion-selective membrane containing a conducting polymer is evaluated. The results obtained with the enzyme field- effect transistor (ENFET) and the ion-selective electrode (ISE) for the determination of creatinine and urea are compared. The presence of the conducting polymer significantly lowers the detection limit for creatinine by one decade to 10−7 and 10−4 M for the ENFET and ISE, respectively. The determination of urea in urine and serum with the ENFET was carried out, and the results correlated well with those obtained by spectrophotometry.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
