A method is described wherein the resolution of P-31 nuclear magnetic resonance spectra of the lipophilic fraction from a Bligh-Dyer extract of rat brain can be enhanced. The lipids are dispersed as micelles in aqueous solution with sodium deoxycholate, and spectral resolution is further optimized by adjusting the pH and by removing ions from the solution. The application of the method to the study of aging in rat brain serves as an example.
Improved resolution of P-31 nuclear magnetic resonance spectra of phospholipids from brain / Capuani, Giorgio; T., Aureli; Miccheli, Alfredo; DI COCCO, Maria Enrica; M. T., Ramacci; Delfini, Maurizio. - In: LIPIDS. - ISSN 0024-4201. - STAMPA. - 27:5(1992), pp. 389-391. [10.1007/BF02536154]
Improved resolution of P-31 nuclear magnetic resonance spectra of phospholipids from brain
CAPUANI, Giorgio;MICCHELI, Alfredo;DI COCCO, Maria Enrica;DELFINI, Maurizio
1992
Abstract
A method is described wherein the resolution of P-31 nuclear magnetic resonance spectra of the lipophilic fraction from a Bligh-Dyer extract of rat brain can be enhanced. The lipids are dispersed as micelles in aqueous solution with sodium deoxycholate, and spectral resolution is further optimized by adjusting the pH and by removing ions from the solution. The application of the method to the study of aging in rat brain serves as an example.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
