Abstract: Aqueous and organic extracts of peripheral human T Iymphocytes and of T lymphoblastoid cell lines have been examinated by P-31 and H-1 NMR spectroscopy in order to study the metabolism of ethanolamine (Etn) linked phosphoglycerides. The results show that the Etn concentration in the culture medium determines the composition of Etn-containing metabolites and phospholipids. The effect of phorbol esters, stimulating the synthesis and the breakdown of choline-containing phospholipids has been also studied. A phorbol 12-myristate 13-acetate (PMA) dependent membrane phosphatidylethanolamine hydrolysis, presumably mediated by protein kinase C activity, has been demonstrated.

P-31 and H-1 studies of ethanolamine-linked phosphoglycerides metabolism in human T Lymphocites / R., Ricciolini; Miccheli, Alfredo; G., Peluso; Delfini, Maurizio; Conti, Filippo. - In: CELLULAR AND MOLECULAR BIOLOGY. - ISSN 0145-5680. - STAMPA. - 37:7(1991), pp. 705-711.

P-31 and H-1 studies of ethanolamine-linked phosphoglycerides metabolism in human T Lymphocites

MICCHELI, Alfredo;DELFINI, Maurizio;CONTI, Filippo
1991

Abstract

Abstract: Aqueous and organic extracts of peripheral human T Iymphocytes and of T lymphoblastoid cell lines have been examinated by P-31 and H-1 NMR spectroscopy in order to study the metabolism of ethanolamine (Etn) linked phosphoglycerides. The results show that the Etn concentration in the culture medium determines the composition of Etn-containing metabolites and phospholipids. The effect of phorbol esters, stimulating the synthesis and the breakdown of choline-containing phospholipids has been also studied. A phorbol 12-myristate 13-acetate (PMA) dependent membrane phosphatidylethanolamine hydrolysis, presumably mediated by protein kinase C activity, has been demonstrated.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/19070
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