Cell wall pectin degradation represents an important step for a successful infection of the host tissue. An important feature of pectin is its degree and pattern of methyl esterification. Highly methyl esterified pectin or a random distribution of methyl ester can be associated with an increased host resistance response. Pectin methyl esterification is controlled by the activity of pectin methylesterase (PME), which de-methylate esterified pectin. Since the activity of PME can be controlled by its inhibitor protein (PMEI), we are characterizing Pmei genes in wheat to manipulate the methyl esterification of pectin and shed light on the involvement of this feature in wheat resistance. We report the characterization of the first Pmei gene (Tdpmei3) in wheat and the isolation of two additional Pmei-like genes (Tdpmei1 and Tdpmei2). qRT-PCR analysis showed that these genes are regulated during leaf development and Tdpmei1 and Tdpmei2 accumulate strongly in the ovary and stamen, whereas Tdpmei3 accumulate mainly in the stem. Tdpmei1 and Tdpmei3 are not induced following wheat leaf infection with the fungal pathogen Bipolaris sorokiniana, whereas Tdpmei2 transcript accumulate slightly.
EXPRESSED PECTIN METHYLESTERASE INHIBITOR (PMEI) GENES SHOW A DIFFERENT PATTERN OF ACCUMULATION IN WHEAT TISSUE AND FOLLOWING FUNGAL INFECTION / Rocchi, V; Janni, M; Kalunke, R; Castiglioni, C; Favaron, F; Bellincampi, Daniela; Giardina, T; D'Ovidio, R.. - (2010), pp. 30-30. (Intervento presentato al convegno Secondo Congresso della Società Italiana di Biologia Vegetale tenutosi a Roma nel 12-14 Luglio 2010).
EXPRESSED PECTIN METHYLESTERASE INHIBITOR (PMEI) GENES SHOW A DIFFERENT PATTERN OF ACCUMULATION IN WHEAT TISSUE AND FOLLOWING FUNGAL INFECTION.
BELLINCAMPI, Daniela;
2010
Abstract
Cell wall pectin degradation represents an important step for a successful infection of the host tissue. An important feature of pectin is its degree and pattern of methyl esterification. Highly methyl esterified pectin or a random distribution of methyl ester can be associated with an increased host resistance response. Pectin methyl esterification is controlled by the activity of pectin methylesterase (PME), which de-methylate esterified pectin. Since the activity of PME can be controlled by its inhibitor protein (PMEI), we are characterizing Pmei genes in wheat to manipulate the methyl esterification of pectin and shed light on the involvement of this feature in wheat resistance. We report the characterization of the first Pmei gene (Tdpmei3) in wheat and the isolation of two additional Pmei-like genes (Tdpmei1 and Tdpmei2). qRT-PCR analysis showed that these genes are regulated during leaf development and Tdpmei1 and Tdpmei2 accumulate strongly in the ovary and stamen, whereas Tdpmei3 accumulate mainly in the stem. Tdpmei1 and Tdpmei3 are not induced following wheat leaf infection with the fungal pathogen Bipolaris sorokiniana, whereas Tdpmei2 transcript accumulate slightly.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
