DNA topoisomerase I together with the other cellular DNA topoisomerases releases the torsional stress from DNA caused by processes such as replication, transcription and recombination. Despite the well-defined knowledge of its mechanism of action, DNA topoisomerase I in vivo activity has been only partially characterized. In fact the basic question concerning the capability of the enzyme to cleave and rejoin DNA wrapped around a histone octamer remains still unanswered. By studying both in vivo and in vitro the cleavage activity of DNA topoisomerase I in the presence of camptothecin on a repeated trinucleotide sequence, (TTA)35, lying in chromosome XIII of Saccharomyces cerevisiae, we can conclude that nucleosomes represent a physical barrier for the enzyme activity. © The Authors.

Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo / DI FELICE, Francesca; Chiani, Francesco; Camilloni, Giorgio. - In: BIOCHEMICAL JOURNAL. - ISSN 0264-6021. - STAMPA. - 409:3(2008), pp. 651-656. [10.1042/bj20070893]

Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo

DI FELICE, Francesca;CHIANI, Francesco;CAMILLONI, Giorgio
2008

Abstract

DNA topoisomerase I together with the other cellular DNA topoisomerases releases the torsional stress from DNA caused by processes such as replication, transcription and recombination. Despite the well-defined knowledge of its mechanism of action, DNA topoisomerase I in vivo activity has been only partially characterized. In fact the basic question concerning the capability of the enzyme to cleave and rejoin DNA wrapped around a histone octamer remains still unanswered. By studying both in vivo and in vitro the cleavage activity of DNA topoisomerase I in the presence of camptothecin on a repeated trinucleotide sequence, (TTA)35, lying in chromosome XIII of Saccharomyces cerevisiae, we can conclude that nucleosomes represent a physical barrier for the enzyme activity. © The Authors.
chromatin; dna structure; dna topoisomerase i; nucleosome; saccharomyces cerevisiae; trinucleotide repeats
01 Pubblicazione su rivista::01a Articolo in rivista
Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo / DI FELICE, Francesca; Chiani, Francesco; Camilloni, Giorgio. - In: BIOCHEMICAL JOURNAL. - ISSN 0264-6021. - STAMPA. - 409:3(2008), pp. 651-656. [10.1042/bj20070893]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/17773
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