Exploring RF-EMF effects on the TRPV4 ion channel across legacy and mmWave frequencies. A molecular dynamics study

The interaction of radiofrequency electromagnetic fields (RF-EMFs) with biological systems is an active area of research, particularly in the transition from legacy communication bands to emerging 5 G millimeter-wave frequencies. In this work, the possible effects of RF-EMF on the TRPV4 ion channel are investigated using an atomistic molecular dynamics approach, comparing representative legacy (900 MHz) and millimeterwave (26 GHz) exposure conditions. Simulations of membraneembedded TRPV4 are performed under controlled RF-EMF exposure to assess frequency-dependent molecular responses. The analysis focuses on structural stability and dynamical behavior of the channel and its surrounding environment. The results indicate that RF exposure does not induce major structural alterations, while frequency-dependent differences in solvent and proteinwater dynamics are observed. This study presents a comparative atomistic framework for examining RF-EMF interactions with TRP channels across different wireless technology generations, revealing frequency-specific dynamical behavior while preserving overall structural stability of the channel.