Laccase from Trametes versicolor was immobilized on Eupergit (R) C250L and alumina particles. The immobilized enzyme was protected by coating with oppositely charged polyelectrolytes by means of the Layer-by-Layer (LbL) technique. Enzymatic assay showed that immobilized laccase was more stable than free enzyme at different pH and temperatures without loss of catalytic efficiency. Immobilized laccase based catalysts were efficiently used for the selective oxidation of alcohols to aldehydes in the presence of redox mediators such as ABTS (2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid), HBT (1-hydroxybenzotriazole), VLA (violuric acid) and TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy free radical)
Synthesis od Aldehydes by Layer by Layer Immobilized laccases in the presence of redox mediators / Guazzaroni, M., Bozzini, T., Saladino, R.. - In: CHEMCATCHEM. - ISSN 1867-3880. - 4:(2012), pp. 1987-1996. [10.1002/cctc.201200330]
Synthesis od Aldehydes by Layer by Layer Immobilized laccases in the presence of redox mediators
Saladino R
2012
Abstract
Laccase from Trametes versicolor was immobilized on Eupergit (R) C250L and alumina particles. The immobilized enzyme was protected by coating with oppositely charged polyelectrolytes by means of the Layer-by-Layer (LbL) technique. Enzymatic assay showed that immobilized laccase was more stable than free enzyme at different pH and temperatures without loss of catalytic efficiency. Immobilized laccase based catalysts were efficiently used for the selective oxidation of alcohols to aldehydes in the presence of redox mediators such as ABTS (2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid), HBT (1-hydroxybenzotriazole), VLA (violuric acid) and TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy free radical)I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
