The treatment of propolis and poplar bud exudates with laccase from Trametes versicolor and TEMPO increased the antioxidant activity, as evaluated by the 2,2’-diphenyl picrylhydrazyl (DPPH) and t-butyl-OOH induced DNA breakage comet assay analyses. The effect was highest for shorter reaction times. Propolis showed the highest antioxidant activity in the DPPH test, while poplar bud exudates were more active in reducing the t-butyl-OOH-induced lesions in CHO cell line. Even if the concentration of polyphenols decreased during the oxidation, the formation of low molecular weight phenols phloroglucinol 4 (1,3,5-trihydroxy benzene), hydroquinone 5 (1,4-dihydroxy benzene), and catechol 6 (1,2-dihydroxybenzene), characterized by radical scavenging activity, can account for the observed increase of the antioxidant activity
Laccase-Mediated Enhancement of the Antioxidant Activity of Propolis and Poplar Bud Exudates / Botta, L., Brunori, F., Tulimieri, A., Piccinino, D., Meschini, R., Saladino, R.. - In: ACS OMEGA. - ISSN 2470-1343. - 2:6(2017), pp. 2515-2523. [10.1021/acsomega.7b00294]
Laccase-Mediated Enhancement of the Antioxidant Activity of Propolis and Poplar Bud Exudates
Saladino R
2017
Abstract
The treatment of propolis and poplar bud exudates with laccase from Trametes versicolor and TEMPO increased the antioxidant activity, as evaluated by the 2,2’-diphenyl picrylhydrazyl (DPPH) and t-butyl-OOH induced DNA breakage comet assay analyses. The effect was highest for shorter reaction times. Propolis showed the highest antioxidant activity in the DPPH test, while poplar bud exudates were more active in reducing the t-butyl-OOH-induced lesions in CHO cell line. Even if the concentration of polyphenols decreased during the oxidation, the formation of low molecular weight phenols phloroglucinol 4 (1,3,5-trihydroxy benzene), hydroquinone 5 (1,4-dihydroxy benzene), and catechol 6 (1,2-dihydroxybenzene), characterized by radical scavenging activity, can account for the observed increase of the antioxidant activityI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
