Bioinformatics-driven, plant-based antibiotic research against quorum sensing and biofilm formation in Pseudomonas aeruginosa and Escherichia coli multiresistant microbes

Quorum-sensing (QS) systems play a crucial role in regulating virulence, biofilm formation, and antibiotic resistance in clinically relevant microbes. This review explores the potential of QS systems as targets for developing novel plant-based therapeutic strategies using bioinformatics, aimed at combating highly pathogenic bacteria: uropathogenic Escherichia coli (UPEC) and Pseudomonas aeruginosa. We examine the key components and molecular pathways of QS systems in these microbes, including autoinducer synthases, receptors, and regulatory proteins. In UPEC, we discuss the LuxS-dependent autoinducer (AI)-2 system, while for P. aeruginosa, we analyze the more complex interconnected Las, Rhl, and PQS circuits. We highlight how these systems control the expression of virulence factors and contribute to biofilm formation, emphasizing their importance in pathogenesis. Furthermore, we explore bioinformatics approaches for identifying and characterizing QS components, i.e., by predicting protein structures and interactions. The potential of in silico screening for QS inhibitors is also discussed, along with challenges and opportunities in targeting QS systems for therapeutic interventions. By integrating microbiological, molecular, and computational perspectives, this review aims to provide insights into the application of bioinformatics in understanding and targeting QS systems in these clinically significant pathogens. The goal is to facilitate the development of novel anti-virulence approaches in search of novel antibiotics that could complement or replace traditional antibiotic treatments, addressing the growing concern of antimicrobial resistance in these clinically relevant microbes.