The oxidation of cysteine to cystine was investigated in aqueous thin films generated by the deposition of electrospray ionization (ESI) microdroplets. The confined volume of the thin film promotes the reaction, resulting in up to 80% conversion of cysteine to cystine at a thin film temperature of 40 °C. The pH of the solution is a critical parameter, influencing both the yield and the kinetics of the reaction. Strong reaction acceleration factors in thin film with respect to the bulk were measured.
Effect of pH on the kinetics of cysteine-to-cystine conversion in thin films formed by ESI microdroplet deposition / Manago, Marta; Salvitti, Chiara; Troiani, Anna; Di Noi, Alessia; Ricci, Andreina; Pepi, Federico. - In: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY. - ISSN 1879-1123. - 36:10(2025), pp. 2189-2196. [10.1021/jasms.5c00195]
Effect of pH on the kinetics of cysteine-to-cystine conversion in thin films formed by ESI microdroplet deposition
marta manago;chiara salvitti;anna troiani;alessia di noi;federico pepi
2025
Abstract
The oxidation of cysteine to cystine was investigated in aqueous thin films generated by the deposition of electrospray ionization (ESI) microdroplets. The confined volume of the thin film promotes the reaction, resulting in up to 80% conversion of cysteine to cystine at a thin film temperature of 40 °C. The pH of the solution is a critical parameter, influencing both the yield and the kinetics of the reaction. Strong reaction acceleration factors in thin film with respect to the bulk were measured.| File | Dimensione | Formato | |
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