This study examined the reproducibility of MYC and BCL-2 immunohistochemical scoring as well as the impact of higher expression of both proteins (double expressor status, DE) on survival and progression in a large retrospective cohort of aggressive B-cell lymphoma patients treated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP) or RCHOP-like regimens with a median follow up of 67 months (range 0-138). We also investigated possible MYC protein expression cut offs with the highest reproducibility among pathologists and predictability of gene translocation. We showed that immunohistochemistry (IHC) for MYC and BCL-2 is highly reproducible when cut-off values of> 70% for MYC and> 50% for BCL-2 are used. This threshold not only predicts the presence of rearrangements (with respect to MYC), but is also clinically valuable. In fact, it identifies a subset of patients …
MYC protein expression scoring and its impact on the prognosis of aggressive B-cell lymphoma patients / R Ambrosio, M., Lazzi, S., Lo Bello, G., Santi, R., Del Porro, L., M De Santi, M., Guazzo, R., Mundo, L., Rigacci, L., Kovalchuck, S., Onyango, N., Fabbri, A., Cencini, E., Luigi Zinzani, P., Zaja, F., Angrilli, F., Stelitano, C., G Cabras, M., Spataro, G., Bob, R., et al.. - In: HAEMATOLOGICA. - ISSN 0390-6078. - (2019). [10.3324/HAEMATOL.2018.195958]
MYC protein expression scoring and its impact on the prognosis of aggressive B-cell lymphoma patients
Lucia Mundo;
2019
Abstract
This study examined the reproducibility of MYC and BCL-2 immunohistochemical scoring as well as the impact of higher expression of both proteins (double expressor status, DE) on survival and progression in a large retrospective cohort of aggressive B-cell lymphoma patients treated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP) or RCHOP-like regimens with a median follow up of 67 months (range 0-138). We also investigated possible MYC protein expression cut offs with the highest reproducibility among pathologists and predictability of gene translocation. We showed that immunohistochemistry (IHC) for MYC and BCL-2 is highly reproducible when cut-off values of> 70% for MYC and> 50% for BCL-2 are used. This threshold not only predicts the presence of rearrangements (with respect to MYC), but is also clinically valuable. In fact, it identifies a subset of patients …I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
