Here, we present a protocol for identifying interactors of circular RNAs (circRNAs), specifically circDlc1(2), in the mouse cortex. We outline steps for tissue dissociation and UV crosslinking to maintain native interactions, followed by an RNA pull-down to isolate the circRNA and its associated molecules. The protocol has been optimized to detect potential protein interactors and can be adapted for use in other regions of the mouse nervous system. For complete details on the use and execution of this protocol, please refer to Silenzi et al.1.
Protocol to study in vivo circRNA interactions in the mouse cortex using an RNA pull-down approach / Silenzi, V.; Salvi, N.; Bozzoni, I.; Morlando, M.. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 6:4(2025). [10.1016/j.xpro.2025.104092]
Protocol to study in vivo circRNA interactions in the mouse cortex using an RNA pull-down approach
Silenzi V.
Primo
;Salvi N.Secondo
;Bozzoni I.Penultimo
;Morlando M.
Ultimo
2025
Abstract
Here, we present a protocol for identifying interactors of circular RNAs (circRNAs), specifically circDlc1(2), in the mouse cortex. We outline steps for tissue dissociation and UV crosslinking to maintain native interactions, followed by an RNA pull-down to isolate the circRNA and its associated molecules. The protocol has been optimized to detect potential protein interactors and can be adapted for use in other regions of the mouse nervous system. For complete details on the use and execution of this protocol, please refer to Silenzi et al.1.| File | Dimensione | Formato | |
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Silenzi_Protocol_2025.pdf
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2.48 MB | Adobe PDF |
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