Salmonella species is an important zoonotic enteropathogen affecting humans and animals. Increasing antibiotic resistance is currently considered a primary issue by World Health Organization (WHO). The purpose of this study was to assess both in silico and in vitro the antimicrobial resistance (AMR) in Salmonella spp. isolates collected in Campania Region from different sources. Thirty-seven draft genomes from Salmonella isolates, belonging to S. Infantis and S. Napoli serotypes, were sequenced using Illumina MiSeq® platform. A total of 68 AMR genes, related with resistance and multidrug resistance were predicted among all isolates. Phenotypic susceptibility to thirteen antimicrobials was determined using minimum inhibitory concentration (MIC). In silico analysis of AMR showed a partial agreement with in vitro assays. Our findings highlight the need of further studies to find uncharacterized genetic determinants related to resistance in vivo.
Valutazione in silico e in vitro della resistenza antimicrobica in ceppi di Salmonella spp. isolati nella regione Campania / Del Sambro, Laura; Simone, Domenico; Bianco, Angelica; Capozzi, Loredana; Barbieri, Giorgia; Quarato, Claudia; Miccolupo, Angela; Corrado, F; Montone, A. M. I.; Parisi, Antonio. - (2021). (Intervento presentato al convegno XX CONGRESSO NAZIONALE S.I.DI.L.V. tenutosi a ON AIR).
Valutazione in silico e in vitro della resistenza antimicrobica in ceppi di Salmonella spp. isolati nella regione Campania
Barbieri, Giorgia;Parisi Antonio
2021
Abstract
Salmonella species is an important zoonotic enteropathogen affecting humans and animals. Increasing antibiotic resistance is currently considered a primary issue by World Health Organization (WHO). The purpose of this study was to assess both in silico and in vitro the antimicrobial resistance (AMR) in Salmonella spp. isolates collected in Campania Region from different sources. Thirty-seven draft genomes from Salmonella isolates, belonging to S. Infantis and S. Napoli serotypes, were sequenced using Illumina MiSeq® platform. A total of 68 AMR genes, related with resistance and multidrug resistance were predicted among all isolates. Phenotypic susceptibility to thirteen antimicrobials was determined using minimum inhibitory concentration (MIC). In silico analysis of AMR showed a partial agreement with in vitro assays. Our findings highlight the need of further studies to find uncharacterized genetic determinants related to resistance in vivo.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
