Background: Functional antibodies (ab) against G protein-coupled receptors (GPCR) were found in various diseases and partially in healthy individuals. Their role in physiology and in pathophysiology remains to be identified. Methods: Sera from 952 patients with different rheumatic diseases were analyzed for the presence of ten different anti-GPCR ab by ELISAs. For deeper ab phenotyping, ab levels against 30 different autoantigens including GPCR, growth factors, and growth factor receptors were studied in sera from 491 healthy controls (HC), 84 patients with systemic sclerosis, 91 with Alzheimer's disease, and 207 with ovarian cancer by ELISAs. Spearman's rank correlation coefficients, hierarchical cluster analyses, network mapping and target interactions using STRING and
Network-based analysis reveals signatures of IgG autoantibodies targeting G protein-coupled receptors in healthy and diseases / Cabral-Marques, O., Marques, A., Giilb, L.M., De Vito, R., Engelhardt, B.E., Sehouli, J., Heidecke H., A.R.G.. - In: EUROPEAN JOURNAL OF IMMUNOLOGY. - ISSN 0014-2980. - (2018).
Network-based analysis reveals signatures of IgG autoantibodies targeting G protein-coupled receptors in healthy and diseases
de Vito R;
2018
Abstract
Background: Functional antibodies (ab) against G protein-coupled receptors (GPCR) were found in various diseases and partially in healthy individuals. Their role in physiology and in pathophysiology remains to be identified. Methods: Sera from 952 patients with different rheumatic diseases were analyzed for the presence of ten different anti-GPCR ab by ELISAs. For deeper ab phenotyping, ab levels against 30 different autoantigens including GPCR, growth factors, and growth factor receptors were studied in sera from 491 healthy controls (HC), 84 patients with systemic sclerosis, 91 with Alzheimer's disease, and 207 with ovarian cancer by ELISAs. Spearman's rank correlation coefficients, hierarchical cluster analyses, network mapping and target interactions using STRING andI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
