Background: Vibrio parahaemolyticus is an emerging foodborne pathogen in the Mediterranean, usually as- sociated with shellfish consumption. The increase in the number of outbreaks in Europe is primarily associated with the global warming of the ocean that has a great impact on the spread and genetic selection of waterborne pathogens. The primary role of Italy in Europe’s mollusk production, together with the fact that cases of infections with V. parahaemolyticus are not always notified to the European community, highlighted the necessity of acquiring new information about the epidemiological involvement of shellfish products. Objective: The aim of the study was to provide useful insights into the first steps of the Risk Assessment associated with V. parahaemolyticus through the molecular characterization of isolates from commercialized mollusks. Materials and Methods: A total of 102 strains identified as V. parahaemolyticus were investigated as part of a larger sampling (1-year survey) from several shellfish species collected from the Venice lagoon and the North Adriatic sea. All strains were characterized by multilocus sequence typing and tested for the presence of virulence genes (trh and tdh). The study of sampling/environmental factors and epidemiological analyses was performed to describe the behaviors of the different genetic populations. Results: The population structure analysis highlighted three genetic clusters that could be subject to temperature selection during cold (£15°C) and warm (>16°C) seasons. Moreover, other factors, such as molluscan species (clams/mussels), probably played a role in the distribution of genetic clusters. Although few strains carried the virulence factors (n=6 trh+), epidemiological links with clinical isolates and a local dissemination of some sequence types were underlined. Conclusion: This work provides a useful background on the genotype spread as a first step in the Hazard Identification in light of future climate changes.
Molecular Typing of Vibrio parahaemolyticus Strains Isolated from Mollusks in the North Adriatic Sea / Shamsur Rahman, Mohammad; Carraro, Roberta; Cardazzo, Barbara; Carraro, Lisa; Boscolo Meneguolo, Davide; Elena Martino, Maria; Andreani, Nadia Andrea; Bordin, Paola; Mioni, Renzo; Barco, Lisa; Novelli, Enrico; Balzan, Stefania; Luca Fasolato, And. - In: FOODBORNE PATHOGENS AND DISEASE. - ISSN 1535-3141. - 14:8(2017), pp. 454-464. [10.1089/fpd.2016.2263]
Molecular Typing of Vibrio parahaemolyticus Strains Isolated from Mollusks in the North Adriatic Sea
Nadia Andrea Andreani;
2017
Abstract
Background: Vibrio parahaemolyticus is an emerging foodborne pathogen in the Mediterranean, usually as- sociated with shellfish consumption. The increase in the number of outbreaks in Europe is primarily associated with the global warming of the ocean that has a great impact on the spread and genetic selection of waterborne pathogens. The primary role of Italy in Europe’s mollusk production, together with the fact that cases of infections with V. parahaemolyticus are not always notified to the European community, highlighted the necessity of acquiring new information about the epidemiological involvement of shellfish products. Objective: The aim of the study was to provide useful insights into the first steps of the Risk Assessment associated with V. parahaemolyticus through the molecular characterization of isolates from commercialized mollusks. Materials and Methods: A total of 102 strains identified as V. parahaemolyticus were investigated as part of a larger sampling (1-year survey) from several shellfish species collected from the Venice lagoon and the North Adriatic sea. All strains were characterized by multilocus sequence typing and tested for the presence of virulence genes (trh and tdh). The study of sampling/environmental factors and epidemiological analyses was performed to describe the behaviors of the different genetic populations. Results: The population structure analysis highlighted three genetic clusters that could be subject to temperature selection during cold (£15°C) and warm (>16°C) seasons. Moreover, other factors, such as molluscan species (clams/mussels), probably played a role in the distribution of genetic clusters. Although few strains carried the virulence factors (n=6 trh+), epidemiological links with clinical isolates and a local dissemination of some sequence types were underlined. Conclusion: This work provides a useful background on the genotype spread as a first step in the Hazard Identification in light of future climate changes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
