A targeted gene signature stratifying mediastinal gray zone lymphoma into classical Hodgkin lymphoma-like or primary mediastinal B-cell lymphoma-like subtypes

In conclusion, aiming at laying the basis for a more accurate stratification of MGZL, we diversified our approach from previous studies3,4 by exploiting computational tools to obtain a restricted gene panel - easily measurable on RNA from formalin-fixed paraffin-embedded samples - which fully separates CHL from PMBL, and molecularly assigns MGZL to one or other of the entities. Such an approach might help in overcoming the histopathological challenge of MGZL categorization, despite efforts to apply the Sarkozy classification to describe proximity to CHL or PMBL. Validation of our signature on larger, independent sets of MGZL would be critical to decipher the underlying relationship between molecular and phenotypic traits, to build a combined histopathological/transcriptomic model of MGZL stratification and, ultimately, to prompt its translation into the clinical setting in order to optimize the treatment of these rare cases.