Therapeutic effect of Marrubium vulgare L. aqueous extract on chronic constriction injury of sciatic nerve-induced neuropathic pain in mice

Marrubium vulgare L. leaves have long been recognised in Moroccan traditional medicine for their effectiveness in managing pain and inflammation. However, their potential is in treating peripheral neuropathic pain. This study aimed to investigate the effects of M. vulgare aqueous extract (AEMV) on peripheral neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve in mice. This research represents the first exploration of AEMV’s therapeutic potential in neuropathic pain management. AEMV was initially analysed using HPLC MS/MS to identify its predominant compound, Marrubiin. Mice underwent CCI surgery to induce neuropathic pain. AEMV was administered orally and Clomipramine served as a positive control. Chronic administration of AEMV significantly attenuated thermal, mechanical, and cold sensitivity induced by CCI. It also promoted functional and histological recovery of the sciatic nerve.