3D bioprinting is at the forefront of tissue engineering to fabricate complex constructs resembling functional tissues. However, the inability to produce heterogeneous tissues and the lack of spatio-temporal control over the release of bioactive factors are greatly limiting clinical translation. Herein, the combination of 3D bioprinting with high-throughput dispensing using a custom microfluidic system and nanoclay-based inks is presented. This approach was found to enhance printability, retention, and controlled release of bioactive factors. Advanced tissue models were developed to resemble cancer and skeletal tissue, while studying the effect of anti-cancer (Doxorubicin) and pro-osteogenic growth factors (bone morphogenetic protein-2, BMP-2), respectively. The engineering of a new nanoclay ink allowed the sustained release, making it suitable for long-term applications. These findings suggest that by combining 3D bioprinting and high-throughput delivery of nanoclay-based inks a new platform for the engineering of functional tissue constructs can be assembled, offering significant advancements in regenerative medicine.

Hybrid 3D microfluidic bioprinting for the engineering of cancer models and tissue substitutes / D’Alessandro, Salvatore; Mohammadi, Sajad; Iafrate, Lucia; Bastioli, Marco; Marcotulli, Martina; Franco, Silvia; Palmisano, Biagio; D’Orazio, Michele; Mencattini, Arianna; Angelini, Roberta; Riminucci, Mara; Marinozzi, Franco; Martinelli, Eugenio; Ruocco, Giancarlo; Bini, Fabiano; Cidonio, Gianluca. - In: VIRTUAL AND PHYSICAL PROTOTYPING. - ISSN 1745-2767. - 19:1(2024). [10.1080/17452759.2024.2419411]

Hybrid 3D microfluidic bioprinting for the engineering of cancer models and tissue substitutes

Lucia Iafrate;Marco Bastioli;Martina Marcotulli;Silvia Franco;Biagio Palmisano;Michele D’Orazio;Mara Riminucci;Franco Marinozzi;Giancarlo Ruocco;Fabiano Bini
;
Gianluca Cidonio
2024

Abstract

3D bioprinting is at the forefront of tissue engineering to fabricate complex constructs resembling functional tissues. However, the inability to produce heterogeneous tissues and the lack of spatio-temporal control over the release of bioactive factors are greatly limiting clinical translation. Herein, the combination of 3D bioprinting with high-throughput dispensing using a custom microfluidic system and nanoclay-based inks is presented. This approach was found to enhance printability, retention, and controlled release of bioactive factors. Advanced tissue models were developed to resemble cancer and skeletal tissue, while studying the effect of anti-cancer (Doxorubicin) and pro-osteogenic growth factors (bone morphogenetic protein-2, BMP-2), respectively. The engineering of a new nanoclay ink allowed the sustained release, making it suitable for long-term applications. These findings suggest that by combining 3D bioprinting and high-throughput delivery of nanoclay-based inks a new platform for the engineering of functional tissue constructs can be assembled, offering significant advancements in regenerative medicine.
2024
3D bioprinting; highthroughput; tissue engineering; gradient; interface
01 Pubblicazione su rivista::01a Articolo in rivista
Hybrid 3D microfluidic bioprinting for the engineering of cancer models and tissue substitutes / D’Alessandro, Salvatore; Mohammadi, Sajad; Iafrate, Lucia; Bastioli, Marco; Marcotulli, Martina; Franco, Silvia; Palmisano, Biagio; D’Orazio, Michele; Mencattini, Arianna; Angelini, Roberta; Riminucci, Mara; Marinozzi, Franco; Martinelli, Eugenio; Ruocco, Giancarlo; Bini, Fabiano; Cidonio, Gianluca. - In: VIRTUAL AND PHYSICAL PROTOTYPING. - ISSN 1745-2767. - 19:1(2024). [10.1080/17452759.2024.2419411]
File allegati a questo prodotto
File Dimensione Formato  
D'Alessandro_Hybrid-3D-microfluidic_2024.pdf

accesso aperto

Note: https://www.tandfonline.com/doi/epdf/10.1080/17452759.2024.2419411?needAccess=true
Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 2.26 MB
Formato Adobe PDF
2.26 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1725309
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact