Human biliary tree stem/progenitor cells (hBTSCs) have been used for cell therapy of patients with advanced liver cirrhosis. Strategies to improve the liver engraftment of transplanted cells are currently under evaluation. We aimed to investigate whether coating hBTSCs with clinical-grade Hyaluronic Acid (HA) may influence their liver engraftment efficiency, proliferation and hepatocyte differentiation.hBTSCs were isolated, by EpCAM-immunoselection, from the extrahepatic biliary tree of adult liver donors and incubated with 0.1% HA. In vitro, HA-coated and uncoated hBTSCs were analyzed for cell viability, colony formation, proliferation and mRNA expression of CD44,ITGβ1,ITGβ4,CDH1. In vivo, HA-coated and uncoated hBTSCs were injected into the spleen of SCID mice. After 30 days, the liver engraftment and ectopic distribution in distant organs were evaluated by immunohistochemistry. The human albumin mRNA into the mice liver was measured by a de novo developed RT-qPCR method and the human albumin concentration in the mice serum was evaluated by ELISA.
A new strategy to improve the liver engraftment efficiency of transplanted human biliary tree stem/progenitor cells (hBTSCs): Cell coating with hyaluronic acid / Nevi, L.; Carpino, G.; Costantini, D.; Cardinale, V.; Riccioni, O.; Di Matteo, S.; Melandro, F.; Berloco, P. B.; Reid, L.; Gaudio, E.; Alvaro, D.. - 49:1(2017), pp. E11-E11. (Intervento presentato al convegno 50th AISF Annual Meeting AISF tenutosi a Rome) [10.1016/j.dld.2017.01.024].
A new strategy to improve the liver engraftment efficiency of transplanted human biliary tree stem/progenitor cells (hBTSCs): Cell coating with hyaluronic acid
Nevi, L.;Carpino, G.;Costantini, D.;Cardinale, V.;Riccioni, O.;Di Matteo, S.;Melandro, F.;Berloco, P. B.;Gaudio, E.;Alvaro, D.
2017
Abstract
Human biliary tree stem/progenitor cells (hBTSCs) have been used for cell therapy of patients with advanced liver cirrhosis. Strategies to improve the liver engraftment of transplanted cells are currently under evaluation. We aimed to investigate whether coating hBTSCs with clinical-grade Hyaluronic Acid (HA) may influence their liver engraftment efficiency, proliferation and hepatocyte differentiation.hBTSCs were isolated, by EpCAM-immunoselection, from the extrahepatic biliary tree of adult liver donors and incubated with 0.1% HA. In vitro, HA-coated and uncoated hBTSCs were analyzed for cell viability, colony formation, proliferation and mRNA expression of CD44,ITGβ1,ITGβ4,CDH1. In vivo, HA-coated and uncoated hBTSCs were injected into the spleen of SCID mice. After 30 days, the liver engraftment and ectopic distribution in distant organs were evaluated by immunohistochemistry. The human albumin mRNA into the mice liver was measured by a de novo developed RT-qPCR method and the human albumin concentration in the mice serum was evaluated by ELISA.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
