Stomach cancer is the 4th most common cancer diag-nosed worldwide. Despite intensive research on its etio-pathology, its treatment strategies have not changed in the last 50 years. Mushrooms have recently attracted much attention as the source of bioactive compounds that can potentially complement cancer therapies. Here, we extracted a phenolic fraction from Lactarius deterri-mus and analyzed its composition and bioactivity against the gastric cancer (AGS) cells. The complexity of L. deter-rimus compounds was revealed by an HPLC assay, and was accompanied by cytostatic, cytotoxic and anti-inva-sive effects of the L. deterrimus extract (LDE). These are illustrated by inhibition of the AGS cells' proliferation, metabolic activity and motility, and by induction of the cytoskeleton rearrangements. Apparently, these effects are exerted via activation of intracellular oxidative stress and decreased ATP production in AGS cells that could not be compensated by induction of autophagy. Less severe LDE effects were seen on physiology of normal gastric fibroblasts; however, inhibition of their motil-ity indicates that LDE can interfere with gastric cancer development via an effect on stromal cells. Along with the observed synergy of LDE and cisplatin/5-fluorouracil effects on AGS cells, our data show the potential of LDE for supplementation of the gastric cancer therapy.
Bioactive compounds from Lactarius deterrimus interfere with the invasive potential of gastric cancer cells / Krol, Kamila; Pudelek, Maciej; Krzysiek-M(\k(a))czka, Gracjana; Wierdak, Mateusz; (z))ena Muszy('(n))ska, Bo(\.; Su(\l)kowska-Ziaja, Katarzyna; Krakowska, Agata; Ryszawy, Damian; (z)), Jaros(\l)aw Czy(\.. - In: ACTA BIOCHIMICA POLONICA. - ISSN 0001-527X. - 68:4(2021), pp. 503-513. [10.18388/abp.2020_5915]
Bioactive compounds from Lactarius deterrimus interfere with the invasive potential of gastric cancer cells
Kamila Krol
;
2021
Abstract
Stomach cancer is the 4th most common cancer diag-nosed worldwide. Despite intensive research on its etio-pathology, its treatment strategies have not changed in the last 50 years. Mushrooms have recently attracted much attention as the source of bioactive compounds that can potentially complement cancer therapies. Here, we extracted a phenolic fraction from Lactarius deterri-mus and analyzed its composition and bioactivity against the gastric cancer (AGS) cells. The complexity of L. deter-rimus compounds was revealed by an HPLC assay, and was accompanied by cytostatic, cytotoxic and anti-inva-sive effects of the L. deterrimus extract (LDE). These are illustrated by inhibition of the AGS cells' proliferation, metabolic activity and motility, and by induction of the cytoskeleton rearrangements. Apparently, these effects are exerted via activation of intracellular oxidative stress and decreased ATP production in AGS cells that could not be compensated by induction of autophagy. Less severe LDE effects were seen on physiology of normal gastric fibroblasts; however, inhibition of their motil-ity indicates that LDE can interfere with gastric cancer development via an effect on stromal cells. Along with the observed synergy of LDE and cisplatin/5-fluorouracil effects on AGS cells, our data show the potential of LDE for supplementation of the gastric cancer therapy.| File | Dimensione | Formato | |
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