: Low-density lipoproteins (LDLs) are the most abundant circulating lipoproteins and the most critical factor in the development of atherosclerosis. This protocol allows the staining of LDLs with oil red O to monitor particle uptake in bright-field microscopy. Here, we describe how to stain isolated LDLs using oil red O and how to use them to monitor LDL uptake in time-lapse experiments or fixed cells.
Protocol for oil red O staining of low-density lipoproteins for in vivo cell treatment / Bini, Simone; Covino, Stella; Minicocci, Ilenia; D'Erasmo, Laura; Tramontano, Daniele; Di Costanzo, Alessia; Arca, Marcello; Pecce, Valeria. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 5:4(2024). [10.1016/j.xpro.2024.103376]
Protocol for oil red O staining of low-density lipoproteins for in vivo cell treatment
Bini, Simone;Covino, Stella;Minicocci, Ilenia;D'Erasmo, Laura;Tramontano, Daniele;Di Costanzo, Alessia;Arca, Marcello;Pecce, Valeria
2024
Abstract
: Low-density lipoproteins (LDLs) are the most abundant circulating lipoproteins and the most critical factor in the development of atherosclerosis. This protocol allows the staining of LDLs with oil red O to monitor particle uptake in bright-field microscopy. Here, we describe how to stain isolated LDLs using oil red O and how to use them to monitor LDL uptake in time-lapse experiments or fixed cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
