The aim of this work is the characterization of the whole monomeric SARS-CoV-2 S protein and its do-mains (S, S1, S2 and RBD) at a serological pH (7.4) by measuring their amide I infrared ab-sorption bands through Attenuated Total Reflectance Infrared spectroscopy (ATR-IR), one of the main and suitable techniques used in the secondary structure analysis of proteins. Experimental data in combination with MultiFOLD predictions, Define Secondary Structure of Proteins (DSSP) web server (DSSP) and Gravy value calculations, provide a comprehensive understanding of proteins’ domains in terms of their secondary structure content, conformational order and interaction with the solvent.
ATR-IR spectroscopy: secondary structure investigation of SARS-CoV-2 spike protein Domains / Mosetti, Rosanna; D’Arco, Annalisa; Mancini, Tiziana; Macis, Salvatore; Della Ventura, Giancarlo; Marcelli, Augusto; Tranfo, Giovanna; Minicozzi, Velia; Luchetti, Nicole; Lupi, Stefano. - (2024). (Intervento presentato al convegno Rome Technopole Spoke 6 - International Young Researcher Workshop tenutosi a Rome).
ATR-IR spectroscopy: secondary structure investigation of SARS-CoV-2 spike protein Domains
Rosanna Mosetti
;Annalisa D’Arco;Tiziana Mancini;Salvatore Macis;Stefano Lupi
2024
Abstract
The aim of this work is the characterization of the whole monomeric SARS-CoV-2 S protein and its do-mains (S, S1, S2 and RBD) at a serological pH (7.4) by measuring their amide I infrared ab-sorption bands through Attenuated Total Reflectance Infrared spectroscopy (ATR-IR), one of the main and suitable techniques used in the secondary structure analysis of proteins. Experimental data in combination with MultiFOLD predictions, Define Secondary Structure of Proteins (DSSP) web server (DSSP) and Gravy value calculations, provide a comprehensive understanding of proteins’ domains in terms of their secondary structure content, conformational order and interaction with the solvent.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
