Biogenic fraction of airborne PM10 which includes bacteria, viruses, fungi and pollens, has been proposed as one of the potential causes of the PM10 toxicity. The present study aimed to pro-vide a comprehensive understanding of the microbial community variations associated to PM10, and their main local sources in the surrounding environment in three urban sites of Rome, characterized by differential pollution rate: green area, residential area and polluted area close to the traffic roads. We combined high-throughput amplicon sequencing of the bacterial 16S rRNA gene and the fungal internal transcribed spacer (ITS) region, with detailed chemical analysis of particulate matter sam-pled from air, paved road surfaces and leaf surfaces of Quercus ilex. Our results demonstrated that bacterial and fungal airborne communities were characterized by the highest alpha-diversity and grouped separately from epiphytic and road dust communities. The reconstruction of source-sink relationships revealed that the resuspension/deposition of road dust from traffic might contribute to the maximum magnitude of microbial exchanges. The relative abundance of extremotolerant microbes was found to be enhanced in epiphytic communities and was associated to a progressively increase of pollution levels as well as opportunistic human pathogenicity in fungal communities.

Diversity and source of airborne microbial communities at differential polluted sites of Rome / Pollegioni, Paola; Mattioni, Claudia; Ristorini, Martina; Occhiuto, Donatella; Canepari, Silvia; Korneykova, Maria V.; Gavrichkova, Olga. - In: ATMOSPHERE. - ISSN 2073-4433. - 13:2(2022). [10.3390/atmos13020224]

Diversity and source of airborne microbial communities at differential polluted sites of Rome

Martina Ristorini;Silvia Canepari;
2022

Abstract

Biogenic fraction of airborne PM10 which includes bacteria, viruses, fungi and pollens, has been proposed as one of the potential causes of the PM10 toxicity. The present study aimed to pro-vide a comprehensive understanding of the microbial community variations associated to PM10, and their main local sources in the surrounding environment in three urban sites of Rome, characterized by differential pollution rate: green area, residential area and polluted area close to the traffic roads. We combined high-throughput amplicon sequencing of the bacterial 16S rRNA gene and the fungal internal transcribed spacer (ITS) region, with detailed chemical analysis of particulate matter sam-pled from air, paved road surfaces and leaf surfaces of Quercus ilex. Our results demonstrated that bacterial and fungal airborne communities were characterized by the highest alpha-diversity and grouped separately from epiphytic and road dust communities. The reconstruction of source-sink relationships revealed that the resuspension/deposition of road dust from traffic might contribute to the maximum magnitude of microbial exchanges. The relative abundance of extremotolerant microbes was found to be enhanced in epiphytic communities and was associated to a progressively increase of pollution levels as well as opportunistic human pathogenicity in fungal communities.
2022
chemical tracers; metabarcoding; microbiomes; particulate matter; pathogenic microorganisms; pollution; urban area
01 Pubblicazione su rivista::01a Articolo in rivista
Diversity and source of airborne microbial communities at differential polluted sites of Rome / Pollegioni, Paola; Mattioni, Claudia; Ristorini, Martina; Occhiuto, Donatella; Canepari, Silvia; Korneykova, Maria V.; Gavrichkova, Olga. - In: ATMOSPHERE. - ISSN 2073-4433. - 13:2(2022). [10.3390/atmos13020224]
File allegati a questo prodotto
File Dimensione Formato  
Pollegioni_Diversity-and-source_2022.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 15.17 MB
Formato Adobe PDF
15.17 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1707904
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 13
  • ???jsp.display-item.citation.isi??? ND
social impact