ThepricklypearisaplantbelongingtotheCactaceaefamily,anditisthebest-knownspeciesthathasgainedsignificantrecognitioninrecentyears.Opuntiasp.cladodeshavebeentraditionallyutilizedasafunctionalfoodandintraditionalmedicinefortreatingchronicdiseases.Therefore,thisstudyaimstoevaluatetheanti-oxidant,antimicrobial,andpharmacologicalactivitiesofcladodeextractsfromOpuntiamicrodasys(Cl1)andOpuntiamacrorhiza(Cl2),aswellastoidentifytheirphytochemicalcompounds.Thechemicalcompositionofthecladodeextractswasanalyzedusingspectrophotometricmethods,whiletheantioxidantactivitywasdeterminedthroughDNAnickingassays.Microdilutionassayswereemployedtoassesstheantimicrobialactivity.Analgesicandanti-inflammatoryactivitiesweredeterminedusingtheaceticacidwrithingtestinmiceandthecarrageenan-inducedWistarratpawedematest.TheresultsrevealedthatthetwoextractsofcladodesfromCl1andCl2werecharacterizedbyahighphenoliccontent(58.13and44.58GAE/gofextract,respectively).Allthetestedextractsexhibitedantimicrobialactivityagainstthestrainstested,withMini-mumInhibitoryConcentration(MIC)valuesrangingfrom0.312to5mg/mLandMinimumBactericidalCon-centration(MBC)valuesrangingfrom1.25to10mg/mL.ThecladodeextractsofCl1andCl2atadoseof300mg/kgshowedadose-dependentinhibitionofaceticacid-inducedwrithinginmice,withrespectiveval-uesof64.76%and61.91%.Themaximumanti-inflammatoryactivityofalldosesofcladodeextractswasobserved5haftercarrageenan-inducedpawedema,andalltesteddosessignificantlyinhibitedtheinducedinflammation.
Phytochemical characterization and biological activities evaluation of Opuntia sp. cladode / Chahdoura, Hassiba; Ben Hsouna, Anis; Ali Boujbiha, Mohamed; Mnif, Wissem; Snoussi, Mejdi; Khemiss, Mehdi; El Bok, Safia; Ben M’hadheb, Manel; Garzoli, Stefania; Mosbah, Habib. - In: SOUTH AFRICAN JOURNAL OF BOTANY. - ISSN 0254-6299. - (2024).
Phytochemical characterization and biological activities evaluation of Opuntia sp. cladode
Stefania Garzoli
Penultimo
;
2024
Abstract
ThepricklypearisaplantbelongingtotheCactaceaefamily,anditisthebest-knownspeciesthathasgainedsignificantrecognitioninrecentyears.Opuntiasp.cladodeshavebeentraditionallyutilizedasafunctionalfoodandintraditionalmedicinefortreatingchronicdiseases.Therefore,thisstudyaimstoevaluatetheanti-oxidant,antimicrobial,andpharmacologicalactivitiesofcladodeextractsfromOpuntiamicrodasys(Cl1)andOpuntiamacrorhiza(Cl2),aswellastoidentifytheirphytochemicalcompounds.Thechemicalcompositionofthecladodeextractswasanalyzedusingspectrophotometricmethods,whiletheantioxidantactivitywasdeterminedthroughDNAnickingassays.Microdilutionassayswereemployedtoassesstheantimicrobialactivity.Analgesicandanti-inflammatoryactivitiesweredeterminedusingtheaceticacidwrithingtestinmiceandthecarrageenan-inducedWistarratpawedematest.TheresultsrevealedthatthetwoextractsofcladodesfromCl1andCl2werecharacterizedbyahighphenoliccontent(58.13and44.58GAE/gofextract,respectively).Allthetestedextractsexhibitedantimicrobialactivityagainstthestrainstested,withMini-mumInhibitoryConcentration(MIC)valuesrangingfrom0.312to5mg/mLandMinimumBactericidalCon-centration(MBC)valuesrangingfrom1.25to10mg/mL.ThecladodeextractsofCl1andCl2atadoseof300mg/kgshowedadose-dependentinhibitionofaceticacid-inducedwrithinginmice,withrespectiveval-uesof64.76%and61.91%.Themaximumanti-inflammatoryactivityofalldosesofcladodeextractswasobserved5haftercarrageenan-inducedpawedema,andalltesteddosessignificantlyinhibitedtheinducedinflammation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
