Plants exploit an immunity-related pectin methylesterase (IT-PME) activity against microbes (Del Corpo et al., 2020). This process could induce the formation of "egg box" structures, resulting in stiffening of the cell wall. IT-PME activity may also promote the production/sensing of damage-associated molecular patterns such as oligogalacturonides and methanol, promoting defensive priming in plants. PME activity can also favour the binding of pathogen recognition receptors such as wall-associated kinases (WAKs), RESISTANCE TO FUSARIUM OXYSPORUM 1 (RFO1) and FERONIA to de-methylesterified pectins. A precise on/off mechanisms regulating PME activity seem necessary for an efficient defense response without compromising growth (Lionetti et al., 2017; Coculo et al., 2022). We revealed that Arabidopsis PMEs involved in plant defense are pro-proteins. We identified two subtilases (SBT), SBT3.3 and SBT3.5, as modulators of IT-PME activity in Arabidopsis immunity to Botrytis. SBT3.3 follows an unconventional secretion pathway to reach the CW where they can activate Pro-PMEs secreted through a conventional protein secretion. The Pro region shares structural similarities with functionally characterized PME inhibitors, mediators of maintenance of CW integrity. Our research reveals the activation of PRO-PMEs as a resistance factor against pathogens. Modification, perception, and signal transduction of pectin methylesterification in plant immunity will be discussed.

Protein precursors for effective plant immunity: the case of pectin methylesterases / Coculo, Daniele; Greco, Marco; DEL CORPO, Daniele; Lionetti, Vincenzo. - (2023). (Intervento presentato al convegno XII Congress of the Italian Society of Plant Biology tenutosi a Bari, Italy).

Protein precursors for effective plant immunity: the case of pectin methylesterases

Daniele Coculo;Marco Greco;Daniele Del Corpo;Vincenzo Lionetti
2023

Abstract

Plants exploit an immunity-related pectin methylesterase (IT-PME) activity against microbes (Del Corpo et al., 2020). This process could induce the formation of "egg box" structures, resulting in stiffening of the cell wall. IT-PME activity may also promote the production/sensing of damage-associated molecular patterns such as oligogalacturonides and methanol, promoting defensive priming in plants. PME activity can also favour the binding of pathogen recognition receptors such as wall-associated kinases (WAKs), RESISTANCE TO FUSARIUM OXYSPORUM 1 (RFO1) and FERONIA to de-methylesterified pectins. A precise on/off mechanisms regulating PME activity seem necessary for an efficient defense response without compromising growth (Lionetti et al., 2017; Coculo et al., 2022). We revealed that Arabidopsis PMEs involved in plant defense are pro-proteins. We identified two subtilases (SBT), SBT3.3 and SBT3.5, as modulators of IT-PME activity in Arabidopsis immunity to Botrytis. SBT3.3 follows an unconventional secretion pathway to reach the CW where they can activate Pro-PMEs secreted through a conventional protein secretion. The Pro region shares structural similarities with functionally characterized PME inhibitors, mediators of maintenance of CW integrity. Our research reveals the activation of PRO-PMEs as a resistance factor against pathogens. Modification, perception, and signal transduction of pectin methylesterification in plant immunity will be discussed.
2023
XII Congress of the Italian Society of Plant Biology
04 Pubblicazione in atti di convegno::04d Abstract in atti di convegno
Protein precursors for effective plant immunity: the case of pectin methylesterases / Coculo, Daniele; Greco, Marco; DEL CORPO, Daniele; Lionetti, Vincenzo. - (2023). (Intervento presentato al convegno XII Congress of the Italian Society of Plant Biology tenutosi a Bari, Italy).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1696992
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