We report on the use of biochips based on one-dimensional photonic crystals sustaining Bloch surface waves to specifically detect target miRNA that is characteristic of hemorrhagic stroke (miR-16-5p) at low concentration in a buffer solution. The biochips were functionalized with streptavidin and ssDNA oligonucleotides to enable miRNA detection. To discriminate the target miRNA from a non-specific control (miR-101a-3p), we made use of an optical platform developed to work both in label-free and fluorescence detection modes. We demonstrate that the limit of detection provided when operating in the fluorescence mode allows us to specifically detect the target miRNA down to 1 ng mL-1 (140 pM), which matches the recommendations for diagnostic miRNA assays, 5 ng mL-1. The low costs open the way towards the application of these disposable optical biochips based on 1DPC sustaining Bloch surface waves as a promising tool for early disease detection in a liquid biopsy format.

Enhanced fluorescence detection of miRNA by means of Bloch surface wave-based biochips / Occhicone, Agostino; Michelotti, Francesco; Rosa, Paola; Chiappetta, Daniele; Pileri, Tommaso; Del Porto, Paola; Danz, Norbert; Munzert, Peter; Pignataro, Giuseppe; Sinibaldi, Alberto. - In: ANALYST. - ISSN 1364-5528. - 148:18(2023), pp. 4429-4437. [10.1039/d3an00804e]

Enhanced fluorescence detection of miRNA by means of Bloch surface wave-based biochips

Occhicone, Agostino
;
Michelotti, Francesco;Rosa, Paola;Chiappetta, Daniele;Pileri, Tommaso;Del Porto, Paola;Sinibaldi, Alberto
2023

Abstract

We report on the use of biochips based on one-dimensional photonic crystals sustaining Bloch surface waves to specifically detect target miRNA that is characteristic of hemorrhagic stroke (miR-16-5p) at low concentration in a buffer solution. The biochips were functionalized with streptavidin and ssDNA oligonucleotides to enable miRNA detection. To discriminate the target miRNA from a non-specific control (miR-101a-3p), we made use of an optical platform developed to work both in label-free and fluorescence detection modes. We demonstrate that the limit of detection provided when operating in the fluorescence mode allows us to specifically detect the target miRNA down to 1 ng mL-1 (140 pM), which matches the recommendations for diagnostic miRNA assays, 5 ng mL-1. The low costs open the way towards the application of these disposable optical biochips based on 1DPC sustaining Bloch surface waves as a promising tool for early disease detection in a liquid biopsy format.
2023
Bloch surface waves; miRNA; sensing
01 Pubblicazione su rivista::01a Articolo in rivista
Enhanced fluorescence detection of miRNA by means of Bloch surface wave-based biochips / Occhicone, Agostino; Michelotti, Francesco; Rosa, Paola; Chiappetta, Daniele; Pileri, Tommaso; Del Porto, Paola; Danz, Norbert; Munzert, Peter; Pignataro, Giuseppe; Sinibaldi, Alberto. - In: ANALYST. - ISSN 1364-5528. - 148:18(2023), pp. 4429-4437. [10.1039/d3an00804e]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1694204
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