Introduction The comparison of effectiveness between R-en-Y gastric bypass (RYGB) and one anastomosis gastric bypass (OAGB/MGB) remains unclear, as well as the changes in gut microbiota (GM). Materials: prospective, cohort, multi-centre study enrolling 96 patients with severe obesity, applying inclusion criteria, randomised between OAGB/MGB or RYGB. Faecal and blood samples were collected, pre- (T0) and 12 months postoperatively (T1). GM was determined by V3-V4 16S rRNA regions sequencing and home-made bioinformatic pipeline based on Qiime2 plugin and R packages. A standardised meal test was performed at T0 and T1 with 250 ml of liquid meal Oxepa (Abbott, Japan). Aim To evaluate and compare OAGB/MGB vs RYGB microbiota profile shift postoperatively and its impact on metabolic and nutritional status. Results 54 patients completed the study, 27 for each procedure (females 61%). No significant differences were found at T0 for anthropometric and nutritional parameters, while a statistically significant anthropometrics’ reduction was registered at T1, with a significant improvement of glucose and lipid profile, and significant reduction in vitamins and iron. Postoperatively fasting glucose, lipid profile, vitamins and minerals were not significantly different between the 2 procedures. Fasting GLP-1 was higher at T1, but postprandial values showed a lower peak both 60' and 120' after ingestion of the mixed meal., with no significant difference between the 2 groups. Examining the GM’s alpha and beta diversity, Firmicutes/Bacteroides ratio increased postoperatively, mainly dependent on Bacteroidetes decreasing. Relative abundances evaluation highlighted those main differences between the identified clusters (group A 29 subjects, group B 14 subjects and group C 10 subjects) were dependent on variation in Bacteroidetes and Firmicutes. A different trend was detected at Phylum level between T0 and T1 of each cluster. Bacteroides and Streptococcus genera appeared mainly changed between the three groups (both showing p< 0.001). Conclusions The emergence of slightly different GM profile postoperatively may be related to clinical condition or may play a key role in the long-term outcome and may represent a biomarker to follow bariatric surgery’s outcomes. No difference between the two bypass techniques were found in GM profile or other examined metabolic parameters one year postoperatively.
IBC-Oxford University2023_BJSOral_2Gut Microbiota and Metabolic Status shift 1 year after Laparoscopic Roux-en-Y vs One Anastomosis Gastric Bypass. Results of a Multi-centre Randomised Controlled Trial / Boru, Cristian Eugeniu; De Maio, F; Capoccia, D; Velotti, N; Verrastro, O; Capaldo, B; Musella, M; Raffaelli, M; Delogu, G; Leonetti, F; Silecchia, G. - In: BRITISH JOURNAL OF SURGERY. - ISSN 0007-1323. - 110:Supplement_9(2023). [10.1093/bjs/znad382.002]
IBC-Oxford University2023_BJSOral_2Gut Microbiota and Metabolic Status shift 1 year after Laparoscopic Roux-en-Y vs One Anastomosis Gastric Bypass. Results of a Multi-centre Randomised Controlled Trial
Boru, Cristian Eugeniu
Writing – Original Draft Preparation
;Capoccia, DMembro del Collaboration Group
;Silecchia, GProject Administration
2023
Abstract
Introduction The comparison of effectiveness between R-en-Y gastric bypass (RYGB) and one anastomosis gastric bypass (OAGB/MGB) remains unclear, as well as the changes in gut microbiota (GM). Materials: prospective, cohort, multi-centre study enrolling 96 patients with severe obesity, applying inclusion criteria, randomised between OAGB/MGB or RYGB. Faecal and blood samples were collected, pre- (T0) and 12 months postoperatively (T1). GM was determined by V3-V4 16S rRNA regions sequencing and home-made bioinformatic pipeline based on Qiime2 plugin and R packages. A standardised meal test was performed at T0 and T1 with 250 ml of liquid meal Oxepa (Abbott, Japan). Aim To evaluate and compare OAGB/MGB vs RYGB microbiota profile shift postoperatively and its impact on metabolic and nutritional status. Results 54 patients completed the study, 27 for each procedure (females 61%). No significant differences were found at T0 for anthropometric and nutritional parameters, while a statistically significant anthropometrics’ reduction was registered at T1, with a significant improvement of glucose and lipid profile, and significant reduction in vitamins and iron. Postoperatively fasting glucose, lipid profile, vitamins and minerals were not significantly different between the 2 procedures. Fasting GLP-1 was higher at T1, but postprandial values showed a lower peak both 60' and 120' after ingestion of the mixed meal., with no significant difference between the 2 groups. Examining the GM’s alpha and beta diversity, Firmicutes/Bacteroides ratio increased postoperatively, mainly dependent on Bacteroidetes decreasing. Relative abundances evaluation highlighted those main differences between the identified clusters (group A 29 subjects, group B 14 subjects and group C 10 subjects) were dependent on variation in Bacteroidetes and Firmicutes. A different trend was detected at Phylum level between T0 and T1 of each cluster. Bacteroides and Streptococcus genera appeared mainly changed between the three groups (both showing p< 0.001). Conclusions The emergence of slightly different GM profile postoperatively may be related to clinical condition or may play a key role in the long-term outcome and may represent a biomarker to follow bariatric surgery’s outcomes. No difference between the two bypass techniques were found in GM profile or other examined metabolic parameters one year postoperatively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.