This work describes the development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care. The electrochemical platform was based on a graphene (GPH) screen-printed electrode (SPE) functionalized by gold-nanospheres (AuNSs) and antibodies against MMP-8 protein (anti-MMP-8). The func- tionalization with anti-MMP-8 was realized by using 11-mercaptoundecanoic acid (11-MUA), thanks to its ability to give strong sulfur bonds with its –SH end, and to cross-link the –NH2 groups of the antibody molecule with the other –COOH end, using the traditional EDC-NHS method. The voltammetric sensor showed good performances with a linear range of 2.5–300 ng mL− 1, a LOD value of 1.0 ± 0.1 ng mL− 1 and a sensitivity of 0.05 μA mL cm− 2 ng− 1. Moreover, the proposed immunosensor was tested in real saliva samples, showing comparable results to those obtained with the conventional ELISA method. The biosensor was single-use and cost-effective and required a small quantity of test medium and a short preparation time, representing a very attractive biosensor for MMP-8 detection in human saliva.

A disposable immunosensor for the detection of salivary MMP-8 as biomarker of periodontitis / Tortolini, Cristina; Gigli, Valeria; Angeloni, Antonio; Tasca, Federico; Thanh, Nguyen T. K.; Antiochia, Riccarda. - In: BIOELECTROCHEMISTRY. - ISSN 1567-5394. - (2023).

A disposable immunosensor for the detection of salivary MMP-8 as biomarker of periodontitis

Cristina Tortolini
Primo
;
Valeria Gigli
Secondo
;
Antonio Angeloni;Federico Tasca;Riccarda Antiochia
Ultimo
2023

Abstract

This work describes the development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care. The electrochemical platform was based on a graphene (GPH) screen-printed electrode (SPE) functionalized by gold-nanospheres (AuNSs) and antibodies against MMP-8 protein (anti-MMP-8). The func- tionalization with anti-MMP-8 was realized by using 11-mercaptoundecanoic acid (11-MUA), thanks to its ability to give strong sulfur bonds with its –SH end, and to cross-link the –NH2 groups of the antibody molecule with the other –COOH end, using the traditional EDC-NHS method. The voltammetric sensor showed good performances with a linear range of 2.5–300 ng mL− 1, a LOD value of 1.0 ± 0.1 ng mL− 1 and a sensitivity of 0.05 μA mL cm− 2 ng− 1. Moreover, the proposed immunosensor was tested in real saliva samples, showing comparable results to those obtained with the conventional ELISA method. The biosensor was single-use and cost-effective and required a small quantity of test medium and a short preparation time, representing a very attractive biosensor for MMP-8 detection in human saliva.
2023
MMP-8; Periodontitis; Immunosensor; Voltammetric biosensor; Gold-nanospheres; Human saliva
01 Pubblicazione su rivista::01a Articolo in rivista
A disposable immunosensor for the detection of salivary MMP-8 as biomarker of periodontitis / Tortolini, Cristina; Gigli, Valeria; Angeloni, Antonio; Tasca, Federico; Thanh, Nguyen T. K.; Antiochia, Riccarda. - In: BIOELECTROCHEMISTRY. - ISSN 1567-5394. - (2023).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1692779
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