: Since the establishment of the Sanger sequencing method, scientists around the world focused their efforts to progress in the field to produce the utmost technology. The introduction of next-generation sequencing (NGS) represents a revolutionary step and promises to lead to massive improvements in our understanding on the role of nucleic acids functions. Cancer research began to use this innovative and highly performing method, and interesting results started to appear in colorectal cancer (CRC) analysis. Several studies produced high-quality data in terms of mutation discovery, especially about actionable or less frequently mutated genes, epigenetics, transcriptomics. Analysis of results is unveiling relevant perspectives aiding to evaluate the response to therapies. Novel evidences have been presented also in other directions such as gut microbiota or CRC circulating tumor cells. However, despite its unquestioned potential, NGS poses some issues calling for additional studies. This review intends to offer a view of the state of the art of NGS applications to CRC through examination of the most important technologies and discussion of recent published results.

Next-generation sequencing: recent applications to the analysis of colorectal cancer / Del Vecchio, Filippo; Mastroiaco, Valentina; Di Marco, Antinisca; Compagnoni, Chiara; Capece, Daria; Zazzeroni, Francesca; Capalbo, Carlo; Alesse, Edoardo; Tessitore, Alessandra. - In: JOURNAL OF TRANSLATIONAL MEDICINE. - ISSN 1479-5876. - 15:1(2017), p. 246. [10.1186/s12967-017-1353-y]

Next-generation sequencing: recent applications to the analysis of colorectal cancer

Capalbo, Carlo;
2017

Abstract

: Since the establishment of the Sanger sequencing method, scientists around the world focused their efforts to progress in the field to produce the utmost technology. The introduction of next-generation sequencing (NGS) represents a revolutionary step and promises to lead to massive improvements in our understanding on the role of nucleic acids functions. Cancer research began to use this innovative and highly performing method, and interesting results started to appear in colorectal cancer (CRC) analysis. Several studies produced high-quality data in terms of mutation discovery, especially about actionable or less frequently mutated genes, epigenetics, transcriptomics. Analysis of results is unveiling relevant perspectives aiding to evaluate the response to therapies. Novel evidences have been presented also in other directions such as gut microbiota or CRC circulating tumor cells. However, despite its unquestioned potential, NGS poses some issues calling for additional studies. This review intends to offer a view of the state of the art of NGS applications to CRC through examination of the most important technologies and discussion of recent published results.
2017
Colorectal cancer; Next-generation sequencing; Precision medicine; Targeted therapy
01 Pubblicazione su rivista::01g Articolo di rassegna (Review)
Next-generation sequencing: recent applications to the analysis of colorectal cancer / Del Vecchio, Filippo; Mastroiaco, Valentina; Di Marco, Antinisca; Compagnoni, Chiara; Capece, Daria; Zazzeroni, Francesca; Capalbo, Carlo; Alesse, Edoardo; Tessitore, Alessandra. - In: JOURNAL OF TRANSLATIONAL MEDICINE. - ISSN 1479-5876. - 15:1(2017), p. 246. [10.1186/s12967-017-1353-y]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1692570
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