In this study, various conventional and innovative methods were investigated for the recovery of polyhydroxyalkanoates (PHA) from a single batch of biomass produced at a pilot scale from mixed microbial cultures (MMCs) and fermented sewage sludge as a feedstock. Sustainable chlorine-free methods using NaOH and/or H2O2, as well as extraction in nontoxic ethyl acetate, were analyzed. Interestingly, the combined treatment of biomass with NaOH and H2O2 solutions demonstrated good recovery (70 wt %) and high purity (92 wt %) of the polymer in small-scale trials. Moreover, when the coupled treatment was performed on a larger biomass quantity (approximately 200 g), it achieved high purity and recovery yield (93 and 88 wt %, respectively), indicating the feasibility of this extraction method on a larger scale.

Chlorine-free Extractions of Mixed-Culture Polyhydroxyalkanoates Produced from Fermented Sewage Sludge at Pilot Scale / Salvatori, Gaia; Alfano, Sara; Martinelli, Andrea; Gottardo, Marco; Villano, Marianna; Ferreira Bruno, Sommer; Valentino, Francesco; Lorini, Laura. - In: INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH. - ISSN 1520-5045. - 62:43(2023), pp. 17400-17407. [10.1021/acs.iecr.3c02684]

Chlorine-free Extractions of Mixed-Culture Polyhydroxyalkanoates Produced from Fermented Sewage Sludge at Pilot Scale

Salvatori Gaia;Alfano Sara;Martinelli Andrea;Villano Marianna;Lorini Laura
2023

Abstract

In this study, various conventional and innovative methods were investigated for the recovery of polyhydroxyalkanoates (PHA) from a single batch of biomass produced at a pilot scale from mixed microbial cultures (MMCs) and fermented sewage sludge as a feedstock. Sustainable chlorine-free methods using NaOH and/or H2O2, as well as extraction in nontoxic ethyl acetate, were analyzed. Interestingly, the combined treatment of biomass with NaOH and H2O2 solutions demonstrated good recovery (70 wt %) and high purity (92 wt %) of the polymer in small-scale trials. Moreover, when the coupled treatment was performed on a larger biomass quantity (approximately 200 g), it achieved high purity and recovery yield (93 and 88 wt %, respectively), indicating the feasibility of this extraction method on a larger scale.
2023
Biomass; biopolymers; extraction; plastics; polymers
01 Pubblicazione su rivista::01a Articolo in rivista
Chlorine-free Extractions of Mixed-Culture Polyhydroxyalkanoates Produced from Fermented Sewage Sludge at Pilot Scale / Salvatori, Gaia; Alfano, Sara; Martinelli, Andrea; Gottardo, Marco; Villano, Marianna; Ferreira Bruno, Sommer; Valentino, Francesco; Lorini, Laura. - In: INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH. - ISSN 1520-5045. - 62:43(2023), pp. 17400-17407. [10.1021/acs.iecr.3c02684]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1692084
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