Chlamydia trachomatis, the main cause of bacterial sexually transmitted diseases, is responsible for severe reproductive sequelae. Amongst all the cytokines involved in host immunity towards this pathogen, IFN-ε has recently acquired importance for its potential contribution to the female reproductive tract innate defenses. Herein, our study aimed to explore, for the first time, the activity of IFN-ε toward C. trachomatis in an in vitro infection model, by testing its effects on the different phases of chlamydial developmental cycle, as well as on the ultrastructural characteristics of chlamydial inclusions, via transmission electron microscopy. Main result is the capability of IFN-ε to alter C. trachomatis growth, as suggested by reduced infectious progenies, as well as a patchy distribution of bacteria and altered morphology of reticulate bodies within inclusions. In conclusion, our results suggest that IFN-ε could play a role in the innate and adaptive immune defenses against C. trachomatis; in the future, it will be needed to investigate its activity on an infection model more closely resembling the physiological environment of the female genital tract.

Interferon-ε as potential inhibitor of Chlamydia trachomatis infection / Filardo, Simone; DI PIETRO, Marisa; Bozzuto, Giuseppina; Fracella, Matteo; Bitossi, Camilla; Molinari, Agnese; Scagnolari, Carolina; Antonelli, Guido; Sessa, Rosa. - In: MICROBIAL PATHOGENESIS. - ISSN 0882-4010. - 185:(2023), pp. 1-6. [10.1016/j.micpath.2023.106427]

Interferon-ε as potential inhibitor of Chlamydia trachomatis infection

Simone Filardo;Marisa Di Pietro;Matteo Fracella;Camilla Bitossi;Carolina Scagnolari;Guido Antonelli;Rosa Sessa
2023

Abstract

Chlamydia trachomatis, the main cause of bacterial sexually transmitted diseases, is responsible for severe reproductive sequelae. Amongst all the cytokines involved in host immunity towards this pathogen, IFN-ε has recently acquired importance for its potential contribution to the female reproductive tract innate defenses. Herein, our study aimed to explore, for the first time, the activity of IFN-ε toward C. trachomatis in an in vitro infection model, by testing its effects on the different phases of chlamydial developmental cycle, as well as on the ultrastructural characteristics of chlamydial inclusions, via transmission electron microscopy. Main result is the capability of IFN-ε to alter C. trachomatis growth, as suggested by reduced infectious progenies, as well as a patchy distribution of bacteria and altered morphology of reticulate bodies within inclusions. In conclusion, our results suggest that IFN-ε could play a role in the innate and adaptive immune defenses against C. trachomatis; in the future, it will be needed to investigate its activity on an infection model more closely resembling the physiological environment of the female genital tract.
2023
anti-bacterial activity; chlamydia trachomatis; genital infections; interferon-ε
01 Pubblicazione su rivista::01a Articolo in rivista
Interferon-ε as potential inhibitor of Chlamydia trachomatis infection / Filardo, Simone; DI PIETRO, Marisa; Bozzuto, Giuseppina; Fracella, Matteo; Bitossi, Camilla; Molinari, Agnese; Scagnolari, Carolina; Antonelli, Guido; Sessa, Rosa. - In: MICROBIAL PATHOGENESIS. - ISSN 0882-4010. - 185:(2023), pp. 1-6. [10.1016/j.micpath.2023.106427]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1691864
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