Transformation products of antibacterial drugs in environmental water: Identification approaches based on liquid chromatography-high resolution mass spectrometry

In recent years, the presence of antibiotics in the aquatic environment has caused increasing concern for the possible consequences on human health and ecosystems, including the development of antibiotic-resistant bacteria. However, once antibiotics enter the environment, mainly through hospital and municipal discharges and the effluents of wastewater treatment plants, they can be subject to transformation reactions, driven by both biotic (e.g. microorganism and mammalian metabolisms) and abiotic factors (e.g. oxidation, photodegradation, and hydrolysis). The resulting transformation products (TPs) can be less or more active than their parent compounds, therefore the inclusion of TPs in monitoring programs should be mandatory. However, only the reference standards of a few known TPs are available, whereas many other TPs are still unknown, due to the high diversity of possible transformation reactions in the environment. Modern high-resolution mass spectrometry (HRMS) instrumentation is now ready to tackle this problem through suspect and untargeted screening approaches. However, for handling the large amount of data typically encountered in the analysis of environmental samples, these approaches also require suitable processing workflows and accurate tandem mass spectra interpretation. The compilation of a suspect list containing the possible monoisotopic masses of TPs retrieved from the literature and/or from laboratory simulated degradation experiments showed unique advantages. However, the employment of in silico prediction tools could improve the identification reliability. In this review, the most recent strategies relying on liquid chromatography-HRMS for the analysis of environmental TPs of the main antibiotic classes were examined, whereas TPs formed during water treatments or disinfection were not included.