Introduction: The use of biocompatible scaffolds combined with the implantation of neural stem cells, is increasingly being investigated to promote the regeneration of damaged neural tissue, for instance, after a Spinal Cord Injury (SCI). In particular, aligned Polylactic Acid (PLA) microfibrils’ scaffolds are capable of supporting cells, promoting their survival and guiding their differentiation in neural lineage to repair the lesion. Despite its biocompatible nature, PLA is an electrically insulating material and thus it could be detrimental for increasingly common scaffolds’ electric functionalization, aimed at accelerating the cellular processes. In this context, the European RISEUP project aims to combine high intense microseconds pulses and DC stimulation with neurogenesis, supported by a PLA microfibrils’ scaffold. Methods: In this paper a numerical study on the effect of microfibrils’ scaffolds on the E-field distribution, in planar interdigitated electrodes, is presented. Realistic microfibrils’ 3D CAD models have been built to carry out a numerical dosimetry study, through Comsol Multiphysics software. Results: Under a voltage of 10 V, microfibrils redistribute the E-field values focalizing the field streamlines in the spaces between the fibers, allowing the field to pass and reach maximum values up to 100 kV/m and values comparable with the bare electrodes’ device (without fibers). Discussion: Globally the median E-field inside the scaffolded electrodes is the 90% of the nominal field, allowing an adequate cells’ exposure.

Electric Field Bridging-Effect in Electrified Microfibrils’ Scaffolds / Fontana, Sara; Caramazza, Laura; Marracino, Paolo; Cuenca Ortolá, Irene; Colella, Micol; Dolciotti, Noemi; Paffi, Alessandra; Gisbert Roca, Fernando; Ivashchenko, Sergiy; Más Estellés, Jorge; Consales, Claudia; Balucani, Marco; Apollonio, Francesca; Liberti, Micaela. - In: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY. - ISSN 2296-4185. - (2023), pp. 1-13. [10.3389/fbioe.2023.1264406]

Electric Field Bridging-Effect in Electrified Microfibrils’ Scaffolds

Sara Fontana;Laura Caramazza;Paolo Marracino;Micol Colella;Noemi Dolciotti;Alessandra Paffi;Marco Balucani;Francesca Apollonio;Micaela Liberti
2023

Abstract

Introduction: The use of biocompatible scaffolds combined with the implantation of neural stem cells, is increasingly being investigated to promote the regeneration of damaged neural tissue, for instance, after a Spinal Cord Injury (SCI). In particular, aligned Polylactic Acid (PLA) microfibrils’ scaffolds are capable of supporting cells, promoting their survival and guiding their differentiation in neural lineage to repair the lesion. Despite its biocompatible nature, PLA is an electrically insulating material and thus it could be detrimental for increasingly common scaffolds’ electric functionalization, aimed at accelerating the cellular processes. In this context, the European RISEUP project aims to combine high intense microseconds pulses and DC stimulation with neurogenesis, supported by a PLA microfibrils’ scaffold. Methods: In this paper a numerical study on the effect of microfibrils’ scaffolds on the E-field distribution, in planar interdigitated electrodes, is presented. Realistic microfibrils’ 3D CAD models have been built to carry out a numerical dosimetry study, through Comsol Multiphysics software. Results: Under a voltage of 10 V, microfibrils redistribute the E-field values focalizing the field streamlines in the spaces between the fibers, allowing the field to pass and reach maximum values up to 100 kV/m and values comparable with the bare electrodes’ device (without fibers). Discussion: Globally the median E-field inside the scaffolded electrodes is the 90% of the nominal field, allowing an adequate cells’ exposure.
2023
tissue engineering; biocompatible scaffold; microfibrils; electric stimulation; numerical modeling, dosimetry
01 Pubblicazione su rivista::01a Articolo in rivista
Electric Field Bridging-Effect in Electrified Microfibrils’ Scaffolds / Fontana, Sara; Caramazza, Laura; Marracino, Paolo; Cuenca Ortolá, Irene; Colella, Micol; Dolciotti, Noemi; Paffi, Alessandra; Gisbert Roca, Fernando; Ivashchenko, Sergiy; Más Estellés, Jorge; Consales, Claudia; Balucani, Marco; Apollonio, Francesca; Liberti, Micaela. - In: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY. - ISSN 2296-4185. - (2023), pp. 1-13. [10.3389/fbioe.2023.1264406]
File allegati a questo prodotto
File Dimensione Formato  
Fontana_Electric_2023.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 3.92 MB
Formato Adobe PDF
3.92 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1691580
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact