Photothermal atomic force microscopy-assisted infrared nanospectroscopy is applied to investigate protein conformation at the nanoscale. In the framework of protein aggregation, the possibility to probe protein conformation on nanometr-sized structures, enabled us to identify structural differences among fibrils formed under different conditions. By adopting a difference IR nanospectroscopy approach (visible light ON-visible light OFF), we then probed, for the first time, subtle transient conformational changes of light-sensitive protein receptors embedded in individual native cell membrane patches.

Mid-Infrared nanospectroscopy to probe protein conformation at the nanoscale / Intze, A.; Temperini, M. E.; Polito, R.; Ortolani, M.; Giliberti, V.. - (2023), pp. 1-3. (Intervento presentato al convegno 2023 48th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz) tenutosi a Montreal, QC, Canada) [10.1109/IRMMW-THz57677.2023.10298886].

Mid-Infrared nanospectroscopy to probe protein conformation at the nanoscale

Intze, A.;Temperini, M. E.;Polito, R.;Ortolani, M.;Giliberti, V.
2023

Abstract

Photothermal atomic force microscopy-assisted infrared nanospectroscopy is applied to investigate protein conformation at the nanoscale. In the framework of protein aggregation, the possibility to probe protein conformation on nanometr-sized structures, enabled us to identify structural differences among fibrils formed under different conditions. By adopting a difference IR nanospectroscopy approach (visible light ON-visible light OFF), we then probed, for the first time, subtle transient conformational changes of light-sensitive protein receptors embedded in individual native cell membrane patches.
2023
2023 48th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz)
04 Pubblicazione in atti di convegno::04d Abstract in atti di convegno
Mid-Infrared nanospectroscopy to probe protein conformation at the nanoscale / Intze, A.; Temperini, M. E.; Polito, R.; Ortolani, M.; Giliberti, V.. - (2023), pp. 1-3. (Intervento presentato al convegno 2023 48th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz) tenutosi a Montreal, QC, Canada) [10.1109/IRMMW-THz57677.2023.10298886].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1691276
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