Photothermal atomic force microscopy-assisted infrared nanospectroscopy is applied to investigate protein conformation at the nanoscale. In the framework of protein aggregation, the possibility to probe protein conformation on nanometr-sized structures, enabled us to identify structural differences among fibrils formed under different conditions. By adopting a difference IR nanospectroscopy approach (visible light ON-visible light OFF), we then probed, for the first time, subtle transient conformational changes of light-sensitive protein receptors embedded in individual native cell membrane patches.
Mid-Infrared nanospectroscopy to probe protein conformation at the nanoscale / Intze, A.; Temperini, M. E.; Polito, R.; Ortolani, M.; Giliberti, V.. - (2023), pp. 1-3. (Intervento presentato al convegno 2023 48th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz) tenutosi a Montreal, QC, Canada) [10.1109/IRMMW-THz57677.2023.10298886].
Mid-Infrared nanospectroscopy to probe protein conformation at the nanoscale
Intze, A.;Temperini, M. E.;Polito, R.;Giliberti, V.
2023
Abstract
Photothermal atomic force microscopy-assisted infrared nanospectroscopy is applied to investigate protein conformation at the nanoscale. In the framework of protein aggregation, the possibility to probe protein conformation on nanometr-sized structures, enabled us to identify structural differences among fibrils formed under different conditions. By adopting a difference IR nanospectroscopy approach (visible light ON-visible light OFF), we then probed, for the first time, subtle transient conformational changes of light-sensitive protein receptors embedded in individual native cell membrane patches.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
