Virus-encoded microRNAs were first reported in the Epstein–Barr virus in 2004. Subsequently, a few hundred viral miRNAs have been identified, mainly in DNA viruses belonging to the herpesviridae family. To date, only 30 viral miRNAs encoded by RNA viruses are reported by miRBase. Since the outbreak of the SARS-CoV-2 pandemic, several studies have predicted and, in some cases, experimentally validated miRNAs originating from the positive strand of the SARS-CoV-2 genome. By integrating NGS data analysis and qRT-PCR approaches, we found that SARS-CoV-2 also encodes for a viral miRNA arising from the minus (antisense) strand of the viral genome, in the region encoding for ORF1ab, herein referred to as SARS-CoV-2-miR-AS1. Our data show that the expression of this microRNA increases in a time course analysis of SARS-CoV-2 infected cells. Furthermore, enoxacin treatment enhances the accumulation of the mature SARS-CoV-2-miR-AS1 in SARS-CoV-2 infected cells, arguing for a Dicer-dependent processing of this small RNA. In silico analysis suggests that SARS-CoV-2-miR-AS1 targets a set of genes which are translationally repressed during SARS-CoV-2 infection. We experimentally validated that SARS-CoV-2-miR-AS1 targets FOS, thus repressing the AP-1 transcription factor activity in human cells.

A microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 Activity / Greco, Francesco; Lorefice, Elisa; Carissimi, Claudia; Laudadio, Ilaria; Ciccosanti, Fabiola; Di Rienzo, Martina; Colavita, Francesca; Meschi, Silvia; Maggi, Fabrizio; Fimia, Gian Maria; Fulci, Valerio. - In: NON-CODING RNA. - ISSN 2311-553X. - 9:3(2023), p. 33. [10.3390/ncrna9030033]

A microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 Activity

Francesco Greco;Elisa Lorefice;Claudia Carissimi;Ilaria Laudadio;Fabiola Ciccosanti;Francesca Colavita;Fabrizio Maggi;Gian Maria Fimia;Valerio Fulci
2023

Abstract

Virus-encoded microRNAs were first reported in the Epstein–Barr virus in 2004. Subsequently, a few hundred viral miRNAs have been identified, mainly in DNA viruses belonging to the herpesviridae family. To date, only 30 viral miRNAs encoded by RNA viruses are reported by miRBase. Since the outbreak of the SARS-CoV-2 pandemic, several studies have predicted and, in some cases, experimentally validated miRNAs originating from the positive strand of the SARS-CoV-2 genome. By integrating NGS data analysis and qRT-PCR approaches, we found that SARS-CoV-2 also encodes for a viral miRNA arising from the minus (antisense) strand of the viral genome, in the region encoding for ORF1ab, herein referred to as SARS-CoV-2-miR-AS1. Our data show that the expression of this microRNA increases in a time course analysis of SARS-CoV-2 infected cells. Furthermore, enoxacin treatment enhances the accumulation of the mature SARS-CoV-2-miR-AS1 in SARS-CoV-2 infected cells, arguing for a Dicer-dependent processing of this small RNA. In silico analysis suggests that SARS-CoV-2-miR-AS1 targets a set of genes which are translationally repressed during SARS-CoV-2 infection. We experimentally validated that SARS-CoV-2-miR-AS1 targets FOS, thus repressing the AP-1 transcription factor activity in human cells.
2023
microRNA; SARS-CoV-2; FOS; AP-1; SARS-CoV-2-miR-AS1
01 Pubblicazione su rivista::01a Articolo in rivista
A microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 Activity / Greco, Francesco; Lorefice, Elisa; Carissimi, Claudia; Laudadio, Ilaria; Ciccosanti, Fabiola; Di Rienzo, Martina; Colavita, Francesca; Meschi, Silvia; Maggi, Fabrizio; Fimia, Gian Maria; Fulci, Valerio. - In: NON-CODING RNA. - ISSN 2311-553X. - 9:3(2023), p. 33. [10.3390/ncrna9030033]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1691165
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