Mycotoxins are secondary metabolites of fungi that contaminate food and feed, and are involved in a series of foodborne illnesses and disorders in humans and animals. The mitigation of mycotoxin content via enzymatic degradation is a strategy to ensure safer food and feed, and to address the forthcoming issues in view of the global trade and sustainability. Nevertheless, the search for active enzymes is still challenging and time-consuming. The in silico analysis may strongly support the research by providing the evidence-based hierarchization of enzymes for a rational design of more effective experimental trials. The present work dealt with the degradation of aflatoxin B-1 and M-1 by laccase enzymes from Trametes versicolor. The enzymes-substrate interaction for various enzyme isoforms was investigated through 3D molecular modeling techniques. Structural differences among the isoforms have been pinpointed, which may cause different patterns of interaction between aflatoxin B-1 and M-1. The possible formation of different products of degradation can be argued accordingly. Moreover, the laccase gamma isoform was identified as the most suitable for protein engineering aimed at ameliorating the substrate specificity. Overall, 3D modeling proved to be an effective analytical tool to assess the enzyme-substrate interaction and provided a solid foothold for supporting the search of degrading enzyme at the early stage.

Degradation of aflatoxins by means of laccases from Trametes versicolor. An in silico insight / Dellafiora, Luca; Galaverna, Gianni; Reverberi, Massimo; Dall'Asta, Chiara. - In: TOXINS. - ISSN 2072-6651. - 9:1(2017). [10.3390/toxins9010017]

Degradation of aflatoxins by means of laccases from Trametes versicolor. An in silico insight

Reverberi, Massimo;
2017

Abstract

Mycotoxins are secondary metabolites of fungi that contaminate food and feed, and are involved in a series of foodborne illnesses and disorders in humans and animals. The mitigation of mycotoxin content via enzymatic degradation is a strategy to ensure safer food and feed, and to address the forthcoming issues in view of the global trade and sustainability. Nevertheless, the search for active enzymes is still challenging and time-consuming. The in silico analysis may strongly support the research by providing the evidence-based hierarchization of enzymes for a rational design of more effective experimental trials. The present work dealt with the degradation of aflatoxin B-1 and M-1 by laccase enzymes from Trametes versicolor. The enzymes-substrate interaction for various enzyme isoforms was investigated through 3D molecular modeling techniques. Structural differences among the isoforms have been pinpointed, which may cause different patterns of interaction between aflatoxin B-1 and M-1. The possible formation of different products of degradation can be argued accordingly. Moreover, the laccase gamma isoform was identified as the most suitable for protein engineering aimed at ameliorating the substrate specificity. Overall, 3D modeling proved to be an effective analytical tool to assess the enzyme-substrate interaction and provided a solid foothold for supporting the search of degrading enzyme at the early stage.
2017
aflatoxins; biotransformation; enzymatic detoxification; food safety; laccase; mild technologies; mycotoxins mitigation
01 Pubblicazione su rivista::01a Articolo in rivista
Degradation of aflatoxins by means of laccases from Trametes versicolor. An in silico insight / Dellafiora, Luca; Galaverna, Gianni; Reverberi, Massimo; Dall'Asta, Chiara. - In: TOXINS. - ISSN 2072-6651. - 9:1(2017). [10.3390/toxins9010017]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1685267
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