: Laser Transmission Spectroscopy (LTS) is an experimental technique able to determine the particle number concentration and the size of colloidal suspensions by a single measurement of the transmittance of a laser beam through the suspension of particles as a function of the wavelength. In this protocol, we show that LTS represents a unique and powerful tool to investigate suspensions of liposomes, where the precise quantification of the number concentration is particularly relevant for the complete definition of the colloidal properties of the suspension. We study a model formulation of Soy-PC:Chol liposomes and we validate LTS results by comparison with High-Performance Liquid Chromatography determination of lipid mass. Then LTS protocols is applied to state-of-art liposomal nanocarrier suspensions. We explain details of data analysis to obtain the particle number concentration by using the Lambert-Beer law and by calculating the extinction cross section, within the framework of Mie theory for spherical vesicles. We also determine the liposome radius and compare it with the hydrodynamic radius measured by Dynamic Light Scattering. As future perspective, we aim to extend LTS analysis to other nanostructures with different geometries and to contribute to the development of new quantitative strategies for the accurate characterization of nanocarriers and other nanoparticles.

Quantification of particle number concentration in liposomal suspensions by Laser Transmission Spectroscopy (LTS) / Sennato, Simona; Sarra, Angelo; Capria, Carlo Panella La; Bombelli, Cecilia; Donati, Enrica; Postorino, Paolo; Bordi, Federico. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 1873-4367. - 222:(2023), pp. 113137-113143. [10.1016/j.colsurfb.2023.113137]

Quantification of particle number concentration in liposomal suspensions by Laser Transmission Spectroscopy (LTS)

Sennato, Simona
Membro del Collaboration Group
;
Capria, Carlo Panella La
Membro del Collaboration Group
;
Bombelli, Cecilia
Membro del Collaboration Group
;
Donati, Enrica
Membro del Collaboration Group
;
Postorino, Paolo
Membro del Collaboration Group
;
Bordi, Federico
Membro del Collaboration Group
2023

Abstract

: Laser Transmission Spectroscopy (LTS) is an experimental technique able to determine the particle number concentration and the size of colloidal suspensions by a single measurement of the transmittance of a laser beam through the suspension of particles as a function of the wavelength. In this protocol, we show that LTS represents a unique and powerful tool to investigate suspensions of liposomes, where the precise quantification of the number concentration is particularly relevant for the complete definition of the colloidal properties of the suspension. We study a model formulation of Soy-PC:Chol liposomes and we validate LTS results by comparison with High-Performance Liquid Chromatography determination of lipid mass. Then LTS protocols is applied to state-of-art liposomal nanocarrier suspensions. We explain details of data analysis to obtain the particle number concentration by using the Lambert-Beer law and by calculating the extinction cross section, within the framework of Mie theory for spherical vesicles. We also determine the liposome radius and compare it with the hydrodynamic radius measured by Dynamic Light Scattering. As future perspective, we aim to extend LTS analysis to other nanostructures with different geometries and to contribute to the development of new quantitative strategies for the accurate characterization of nanocarriers and other nanoparticles.
2023
Counting technique; Extinction coefficient; Laser Transmission Spectroscopy; Liposomes; Particle number concentration
01 Pubblicazione su rivista::01a Articolo in rivista
Quantification of particle number concentration in liposomal suspensions by Laser Transmission Spectroscopy (LTS) / Sennato, Simona; Sarra, Angelo; Capria, Carlo Panella La; Bombelli, Cecilia; Donati, Enrica; Postorino, Paolo; Bordi, Federico. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 1873-4367. - 222:(2023), pp. 113137-113143. [10.1016/j.colsurfb.2023.113137]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1684067
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