Laccase from white-rot fungi was immobilized onto delignified spent grain by the H2SO4/NaOH digestion. Characteristics of biocatalyst were evaluated by scanning electronic microscope and by kinetic parameters (Km and V-max) after the optimization of immobilization conditions (pH 3, 4 degrees C, 24 h and the ratio of initial activity laccase on support 0.2 U/mg). The biocatalyst was used in batch and with recirculation for the decolourization of three dyes: methyl orange (15 mg/L), murexide (50 mg/L) and methylene blue (10 mg/L) at pH 5 and 30 degrees C. The decolourization rate was accelerated when 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonicacid)dia-mmoniumsalt mediator was added to the mixture. Our results showed that the immobilized laccase on delignified spent grain, a by-product of the brewing industry, could be efficiently used for the removal of synthetic dyes from industrial effluents and could be an appealing alternative to conventional treatments.

Sustainable recycling of spent grain for laccase immobilization as dyes removal tool / Girelli, A. M.; Pambianco, E.; Scuto, F. R.. - In: JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING. - ISSN 2213-3437. - 9:6(2021), pp. 106653-106653. [10.1016/j.jece.2021.106653]

Sustainable recycling of spent grain for laccase immobilization as dyes removal tool

A. M. Girelli
;
F. R. Scuto
2021

Abstract

Laccase from white-rot fungi was immobilized onto delignified spent grain by the H2SO4/NaOH digestion. Characteristics of biocatalyst were evaluated by scanning electronic microscope and by kinetic parameters (Km and V-max) after the optimization of immobilization conditions (pH 3, 4 degrees C, 24 h and the ratio of initial activity laccase on support 0.2 U/mg). The biocatalyst was used in batch and with recirculation for the decolourization of three dyes: methyl orange (15 mg/L), murexide (50 mg/L) and methylene blue (10 mg/L) at pH 5 and 30 degrees C. The decolourization rate was accelerated when 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonicacid)dia-mmoniumsalt mediator was added to the mixture. Our results showed that the immobilized laccase on delignified spent grain, a by-product of the brewing industry, could be efficiently used for the removal of synthetic dyes from industrial effluents and could be an appealing alternative to conventional treatments.
2021
Laccase; spent grain material; enzyme immobilization, dyes decolourization
01 Pubblicazione su rivista::01a Articolo in rivista
Sustainable recycling of spent grain for laccase immobilization as dyes removal tool / Girelli, A. M.; Pambianco, E.; Scuto, F. R.. - In: JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING. - ISSN 2213-3437. - 9:6(2021), pp. 106653-106653. [10.1016/j.jece.2021.106653]
File allegati a questo prodotto
File Dimensione Formato  
Girelli_Sustainable_2021.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.67 MB
Formato Adobe PDF
1.67 MB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1683619
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 12
social impact