All coronaviruses are characterized by spike glycoproteins whose S1 subunits contain the receptor binding domain (RBD). The RBD anchors the virus to the host cellular membrane to regulate the virus transmissibility and infectious process. Although the protein/receptor interaction mainly depends on the spike’s conformation, particularly on its S1 unit, their secondary structures are poorly known. In this paper, the S1 conformation was investigated for MERS-CoV, SARS-CoV, and SARS-CoV-2 at serological pH by measuring their Amide I infrared absorption bands. The SARS-CoV-2 S1 secondary structure revealed a strong difference compared to those of MERS-CoV and SARS-CoV, with a significant presence of extended β-sheets. Furthermore, the conformation of the SARS-CoV-2 S1 showed a significant change by moving from serological pH to mild acidic and alkaline pH conditions. Both results suggest the capability of infrared spectroscopy to follow the secondary structure adaptation of the SARS-CoV-2 S1 to different environments.

Secondary Structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 Spike Proteins Revealed by Infrared Vibrational Spectroscopy / D’Arco, Annalisa; Di Fabrizio, Marta; Mancini, Tiziana; Mosetti, Rosanna; Macis, Salvatore; Tranfo, Giovanna; Della Ventura, Giancarlo; Marcelli, Augusto; Petrarca, Massimo; Lupi, Stefano. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:11(2023). [10.3390/ijms24119550]

Secondary Structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 Spike Proteins Revealed by Infrared Vibrational Spectroscopy

D’Arco, Annalisa
;
Mancini, Tiziana
;
Mosetti, Rosanna;Macis, Salvatore;Petrarca, Massimo;Lupi, Stefano
2023

Abstract

All coronaviruses are characterized by spike glycoproteins whose S1 subunits contain the receptor binding domain (RBD). The RBD anchors the virus to the host cellular membrane to regulate the virus transmissibility and infectious process. Although the protein/receptor interaction mainly depends on the spike’s conformation, particularly on its S1 unit, their secondary structures are poorly known. In this paper, the S1 conformation was investigated for MERS-CoV, SARS-CoV, and SARS-CoV-2 at serological pH by measuring their Amide I infrared absorption bands. The SARS-CoV-2 S1 secondary structure revealed a strong difference compared to those of MERS-CoV and SARS-CoV, with a significant presence of extended β-sheets. Furthermore, the conformation of the SARS-CoV-2 S1 showed a significant change by moving from serological pH to mild acidic and alkaline pH conditions. Both results suggest the capability of infrared spectroscopy to follow the secondary structure adaptation of the SARS-CoV-2 S1 to different environments.
2023
ATR-IR spectroscopy; spike glycoproteins; secondary structure; conformation; SARS-CoV; MERS-CoV; SARS-CoV-2
01 Pubblicazione su rivista::01a Articolo in rivista
Secondary Structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 Spike Proteins Revealed by Infrared Vibrational Spectroscopy / D’Arco, Annalisa; Di Fabrizio, Marta; Mancini, Tiziana; Mosetti, Rosanna; Macis, Salvatore; Tranfo, Giovanna; Della Ventura, Giancarlo; Marcelli, Augusto; Petrarca, Massimo; Lupi, Stefano. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:11(2023). [10.3390/ijms24119550]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1682362
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