The present Study is designed to investigate the effect of some natural prenyloxypherylpropanoids as neuroprotective agents against NMDA-induced toxicity in mixed cortical cell Cultures containing both neurons and astrocytes. Excitotoxicity was induced by exposure of cultures to NMDA (100 mu M) at room temperature in a HEPES-buffered salt Solution followed by incubation at 37 degrees C for the following 24 h in MEM-Eagle's supplemented with 15.8 mM NaHCO3 and 25 mM glucose. Tested compounds were mixed with NMDA. Neuronal injury was measured in all experiments by examination Of Cultures with phase-contrast microscopy at 20x, 18-20 h after the insult while neuronal damage was quantitatively assessed by counting dead neurons stained with trypan blue and by measuring lactate dehydrogenase (LDH) released in the medium. Results showed that only natural prenyloxypherylpropanoids containing a coumarin nucleus, namely 7-isopentenyloxycounnarin and auraptene, both found in nature from plants belonging to the genus Citrus and other of the family of Rutaceae, including edible ones, exerted a good dose-dependent manner protective effect against NMDA-induced neurotoxicity in particular at concentrations ranging from 1 to 10 mu M. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

Neuroprotective effect of prenyloxycoumarins from edible vegetables / Francesco, Epifano; Gemma, Molinaro; Salvatore, Genovese; Richard Teke, Ngomba; Nicoletti, Ferdinando; Massimo, Curini. - In: NEUROSCIENCE LETTERS. - ISSN 0304-3940. - 443:2(2008), pp. 57-60. [10.1016/j.neulet.2008.07.062]

Neuroprotective effect of prenyloxycoumarins from edible vegetables

NICOLETTI, Ferdinando;
2008

Abstract

The present Study is designed to investigate the effect of some natural prenyloxypherylpropanoids as neuroprotective agents against NMDA-induced toxicity in mixed cortical cell Cultures containing both neurons and astrocytes. Excitotoxicity was induced by exposure of cultures to NMDA (100 mu M) at room temperature in a HEPES-buffered salt Solution followed by incubation at 37 degrees C for the following 24 h in MEM-Eagle's supplemented with 15.8 mM NaHCO3 and 25 mM glucose. Tested compounds were mixed with NMDA. Neuronal injury was measured in all experiments by examination Of Cultures with phase-contrast microscopy at 20x, 18-20 h after the insult while neuronal damage was quantitatively assessed by counting dead neurons stained with trypan blue and by measuring lactate dehydrogenase (LDH) released in the medium. Results showed that only natural prenyloxypherylpropanoids containing a coumarin nucleus, namely 7-isopentenyloxycounnarin and auraptene, both found in nature from plants belonging to the genus Citrus and other of the family of Rutaceae, including edible ones, exerted a good dose-dependent manner protective effect against NMDA-induced neurotoxicity in particular at concentrations ranging from 1 to 10 mu M. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
2008
citrus spp.; mixed cortical cultures; neuroprotective activity; prenyloxycoumarins
01 Pubblicazione su rivista::01a Articolo in rivista
Neuroprotective effect of prenyloxycoumarins from edible vegetables / Francesco, Epifano; Gemma, Molinaro; Salvatore, Genovese; Richard Teke, Ngomba; Nicoletti, Ferdinando; Massimo, Curini. - In: NEUROSCIENCE LETTERS. - ISSN 0304-3940. - 443:2(2008), pp. 57-60. [10.1016/j.neulet.2008.07.062]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/16823
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