The tripartite complex AcrAB-TolC is the major RND pump in Escherichia coli and other Enterobacteriaceae, including Shigella, the etiological agent of bacillary dysentery. In addition to conferring resistance to many classes of antibiotics, AcrAB plays a role in the pathogenesis and virulence of several bacterial pathogens. Here, we report data demonstrating that AcrAB specifically contributes to Shigella flexneri invasion of epithelial cells. We found that deletion of both acrA and acrB genes causes reduced survival of S. flexneri M90T strain within Caco-2 epithelial cells and prevents cell-to-cell spread of the bacteria. Infections with single deletion mutant strains indicate that both AcrA and AcrB favor the viability of the intracellular bacteria. Finally, we were able to further confirm the requirement of the AcrB transporter activity for intraepithelial survival by using a specific EP inhibitor. Overall, the data from the present study expand the role of the AcrAB pump to an important human pathogen, such as Shigella, and add insights into the mechanism governing the Shigella infection process.

Role of the MDR Efflux Pump AcrAB in Epithelial Cell Invasion by Shigella flexneri / Coluccia, Marco; Béranger, Aude; Trirocco, Rita; Fanelli, Giulia; Zanzi, Francesco; Colonna, Bianca; Grossi, Milena; Prosseda, Gianni; Pasqua, Martina. - In: BIOMOLECULES. - ISSN 2218-273X. - 13:5(2023). [10.3390/biom13050823]

Role of the MDR Efflux Pump AcrAB in Epithelial Cell Invasion by Shigella flexneri

Marco Coluccia;Rita Trirocco;Giulia Fanelli;Bianca Colonna;Milena Grossi;Gianni Prosseda;Martina Pasqua
2023

Abstract

The tripartite complex AcrAB-TolC is the major RND pump in Escherichia coli and other Enterobacteriaceae, including Shigella, the etiological agent of bacillary dysentery. In addition to conferring resistance to many classes of antibiotics, AcrAB plays a role in the pathogenesis and virulence of several bacterial pathogens. Here, we report data demonstrating that AcrAB specifically contributes to Shigella flexneri invasion of epithelial cells. We found that deletion of both acrA and acrB genes causes reduced survival of S. flexneri M90T strain within Caco-2 epithelial cells and prevents cell-to-cell spread of the bacteria. Infections with single deletion mutant strains indicate that both AcrA and AcrB favor the viability of the intracellular bacteria. Finally, we were able to further confirm the requirement of the AcrB transporter activity for intraepithelial survival by using a specific EP inhibitor. Overall, the data from the present study expand the role of the AcrAB pump to an important human pathogen, such as Shigella, and add insights into the mechanism governing the Shigella infection process.
2023
Shigella; AcrAB; efflux pumps; microbe–host cell interaction
01 Pubblicazione su rivista::01a Articolo in rivista
Role of the MDR Efflux Pump AcrAB in Epithelial Cell Invasion by Shigella flexneri / Coluccia, Marco; Béranger, Aude; Trirocco, Rita; Fanelli, Giulia; Zanzi, Francesco; Colonna, Bianca; Grossi, Milena; Prosseda, Gianni; Pasqua, Martina. - In: BIOMOLECULES. - ISSN 2218-273X. - 13:5(2023). [10.3390/biom13050823]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1679484
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