We show that in S. cerevisiae the metabolic diauxic shift is associated with a H3 lysine 4 tri-methylation (H3K4me3) increase which involves a significant fraction of transcriptionally induced genes which are required for the metabolic changes, suggesting a role for histone methylation in their transcriptional regulation. We show that histone H3K4me3 around the start site correlates with transcriptional induction in some of these genes. Among the methylation-induced genes are IDP2 and ODC1, which regulate the nuclear availability of α-ketoglutarate, which, as a cofactor for Jhd2 demethylase, regulates H3K4 tri-methylation. We propose that this feedback circuit could be used to regulate the nuclear α-ketoglutarate pool concentration. We also show that yeast cells adapt to the absence of Jhd2 by decreasing Set1 methylation activity.

H3 Lysine 4 Methylation Is Required for Full Activation of Genes Involved in α-Ketoglutarate Availability in the Nucleus of Yeast Cells after Diauxic Shift / DI NISIO, Elena; Danovska, Svetlana; Condemi, Livia; Cirigliano, Angela; Rinaldi, Teresa; Licursi, Valerio; Negri, Rodolfo. - In: METABOLITES. - ISSN 2218-1989. - 13:4(2023). [10.3390/metabo13040507]

H3 Lysine 4 Methylation Is Required for Full Activation of Genes Involved in α-Ketoglutarate Availability in the Nucleus of Yeast Cells after Diauxic Shift

Elena Di Nisio
Primo
Conceptualization
;
Svetlana Danovska
Secondo
Investigation
;
Angela Cirigliano
Investigation
;
Teresa Rinaldi
Investigation
;
Valerio Licursi
Penultimo
Data Curation
;
Rodolfo Negri
Ultimo
Supervision
2023

Abstract

We show that in S. cerevisiae the metabolic diauxic shift is associated with a H3 lysine 4 tri-methylation (H3K4me3) increase which involves a significant fraction of transcriptionally induced genes which are required for the metabolic changes, suggesting a role for histone methylation in their transcriptional regulation. We show that histone H3K4me3 around the start site correlates with transcriptional induction in some of these genes. Among the methylation-induced genes are IDP2 and ODC1, which regulate the nuclear availability of α-ketoglutarate, which, as a cofactor for Jhd2 demethylase, regulates H3K4 tri-methylation. We propose that this feedback circuit could be used to regulate the nuclear α-ketoglutarate pool concentration. We also show that yeast cells adapt to the absence of Jhd2 by decreasing Set1 methylation activity.
2023
H3K4 tri-methylation; diauxic shift; transcriptional regulation
01 Pubblicazione su rivista::01a Articolo in rivista
H3 Lysine 4 Methylation Is Required for Full Activation of Genes Involved in α-Ketoglutarate Availability in the Nucleus of Yeast Cells after Diauxic Shift / DI NISIO, Elena; Danovska, Svetlana; Condemi, Livia; Cirigliano, Angela; Rinaldi, Teresa; Licursi, Valerio; Negri, Rodolfo. - In: METABOLITES. - ISSN 2218-1989. - 13:4(2023). [10.3390/metabo13040507]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1678489
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