: Attention-deficit/hyperactivity disorder (ADHD), a neuropsychiatric condition characterized by inattention, hyperactivity, and impulsivity, afflicts 5% of children worldwide. Each ADHD patient presents with individual cognitive and motivational peculiarities. Furthermore, choice of appropriate therapy is still up to clinicians, who express somewhat qualitative advice on whether a child is being successfully cured or not: it would be more appropriate to use an objective biomarker to indicate whether a treatment led to benefits or not. The aim of our work is to search for such clinical biomarkers. We recruited 60 ADHD kids; psychopathological scales were administered at recruitment and after six weeks of therapy. Out of such a cohort of ADHD children, we rigorously extracted two specific subgroups; regardless of the initial severity of their disease, we compared those who obtained the largest improvement (ΔCGAS > 5) vs. those who were still characterized by a severe condition (CGAS < 40). After such a therapy, methylation levels of DNA extracted from buccal swabs were measured in the 5’-UTR of the DAT1 gene. CpGs 3 and 5 displayed, in relation to the other CpGs, a particular symmetrical pattern; for “improving” ADHD children, they were methylated together with CpG 2 and CpG 6; instead, for “severe” ADHD children, they accompanied a methylated CpG 1. These specific patterns of methylation could be used as objective molecular biomarkers of successful cures, establishing if a certain therapy is akin to a given patient (personalized medicine). Present data support the use of post-therapy molecular data obtained with non-invasive techniques

Methylation dynamics on 5′-UTR of DAT1 gene as a bio-marker to recognize therapy success in ADHD children / Carpentieri, Valentina; Lambacher, Gabriella; Troianiello, Miriam; Pucci, Mariangela; Di Pietro, Diana Di; Laviola, Giovanni; D’Addario, Claudio; Pascale, Esterina; Adriani, Walter. - In: CHILDREN. - ISSN 2227-9067. - 10:3(2023), pp. 1-11. [10.3390/children10030584]

Methylation dynamics on 5′-UTR of DAT1 gene as a bio-marker to recognize therapy success in ADHD children

Di Pietro, Diana Di;Pascale, Esterina;
2023

Abstract

: Attention-deficit/hyperactivity disorder (ADHD), a neuropsychiatric condition characterized by inattention, hyperactivity, and impulsivity, afflicts 5% of children worldwide. Each ADHD patient presents with individual cognitive and motivational peculiarities. Furthermore, choice of appropriate therapy is still up to clinicians, who express somewhat qualitative advice on whether a child is being successfully cured or not: it would be more appropriate to use an objective biomarker to indicate whether a treatment led to benefits or not. The aim of our work is to search for such clinical biomarkers. We recruited 60 ADHD kids; psychopathological scales were administered at recruitment and after six weeks of therapy. Out of such a cohort of ADHD children, we rigorously extracted two specific subgroups; regardless of the initial severity of their disease, we compared those who obtained the largest improvement (ΔCGAS > 5) vs. those who were still characterized by a severe condition (CGAS < 40). After such a therapy, methylation levels of DNA extracted from buccal swabs were measured in the 5’-UTR of the DAT1 gene. CpGs 3 and 5 displayed, in relation to the other CpGs, a particular symmetrical pattern; for “improving” ADHD children, they were methylated together with CpG 2 and CpG 6; instead, for “severe” ADHD children, they accompanied a methylated CpG 1. These specific patterns of methylation could be used as objective molecular biomarkers of successful cures, establishing if a certain therapy is akin to a given patient (personalized medicine). Present data support the use of post-therapy molecular data obtained with non-invasive techniques
2023
dopamine transporter (DAT1) gene; attention-deficit/hyperactivity disorder (ADHD); problematic children; CpGs methylation pattern; methylphenidate (MPH); cognitive-behavioral therapy (CBT)
01 Pubblicazione su rivista::01a Articolo in rivista
Methylation dynamics on 5′-UTR of DAT1 gene as a bio-marker to recognize therapy success in ADHD children / Carpentieri, Valentina; Lambacher, Gabriella; Troianiello, Miriam; Pucci, Mariangela; Di Pietro, Diana Di; Laviola, Giovanni; D’Addario, Claudio; Pascale, Esterina; Adriani, Walter. - In: CHILDREN. - ISSN 2227-9067. - 10:3(2023), pp. 1-11. [10.3390/children10030584]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1675017
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