Unlike other positive-stranded RNA viruses that use either a 5'-cap structure or an internal ribosome entry site to direct translation of their messenger RNA, calicivirus translation is dependent on the presence of a protein covalently linked to the 50 end of the viral genome (VPg). We have shown a direct interaction of the calicivirus VPg with the cap-binding protein eIF4E. This interaction is required for calicivirus mRNA translation, as sequestration of eIF4E by 4E-BP1 inhibits translation. Functional analysis has shown that VPg does not interfere with the interaction between eIF4E and the cap structure or 4E-BP1, suggesting that VPg binds to eIF4E at a different site from both cap and 4E-BP1. This work lends support to the idea that calicivirus VPg acts as a novel 'cap substitute' during initiation of translation on virus mRNA.

Calicivirus translation initiation requires an interaction between VPg and eIF 4 E / Goodfellow, Ian; Chaudhry, Yasmin; Gioldasi, Ioanna; Gerondopoulos, Andreas; Natoni, Alessandro; Labrie, Louisette; Laliberté, Jean-François; Roberts, Lisa. - In: EMBO REPORTS. - ISSN 1469-221X. - 6:10(2005), pp. 968-972. [10.1038/sj.embor.7400510]

Calicivirus translation initiation requires an interaction between VPg and eIF 4 E

Natoni, Alessandro
Investigation
;
2005

Abstract

Unlike other positive-stranded RNA viruses that use either a 5'-cap structure or an internal ribosome entry site to direct translation of their messenger RNA, calicivirus translation is dependent on the presence of a protein covalently linked to the 50 end of the viral genome (VPg). We have shown a direct interaction of the calicivirus VPg with the cap-binding protein eIF4E. This interaction is required for calicivirus mRNA translation, as sequestration of eIF4E by 4E-BP1 inhibits translation. Functional analysis has shown that VPg does not interfere with the interaction between eIF4E and the cap structure or 4E-BP1, suggesting that VPg binds to eIF4E at a different site from both cap and 4E-BP1. This work lends support to the idea that calicivirus VPg acts as a novel 'cap substitute' during initiation of translation on virus mRNA.
2005
calicivirus; eIF4E; translation; VPg
01 Pubblicazione su rivista::01a Articolo in rivista
Calicivirus translation initiation requires an interaction between VPg and eIF 4 E / Goodfellow, Ian; Chaudhry, Yasmin; Gioldasi, Ioanna; Gerondopoulos, Andreas; Natoni, Alessandro; Labrie, Louisette; Laliberté, Jean-François; Roberts, Lisa. - In: EMBO REPORTS. - ISSN 1469-221X. - 6:10(2005), pp. 968-972. [10.1038/sj.embor.7400510]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1673700
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