Idiopathic pulmonary fibrosis (IPF) is a disease characterized by progressive scarring of the lung that involves the pulmonary interstitium. The disease may rapidly progress, leading to respiratory failure, and the long-term survival is poor. There are no accurate biomarkers available so far. Our aim was to evaluate the expression of the B4GALT1 in patients with IPF. Analysis of B4GALT1 gene expression was performed in silico on two gene sets, retrieved from the Gene Expression Omnibus database. Expression of B4GALT1 was then evaluated, both at the mRNA and protein levels, on lung specimens obtained from lung biopsies of 4 IPF patients, on one IPF-derived human primary cell and on 11 cases of IPF associated with cancer. In silico re-analysis demonstrated that the B4GALT1 gene was overexpressed in patients and human cell cultures with IPF (p = 0.03). Network analysis demonstrated that B4GALT1 upregulation was correlated with genes belonging to the EMT pathway (p = 0.01). The overexpression of B4GALT1 was observed, both at mRNA and protein levels, in lung biopsies of our four IPF patients and in the IPF-derived human primary cell, in other fibrotic non-lung tissues, and in IPF associated with cancer. In conclusion, our results indicate that B4GALT1 is overexpressed in IPF and could represent a novel marker of this disease.

B4GALT1 as a New Biomarker of Idiopathic Pulmonary Fibrosis / DE VITIS, Claudia; Michela, ; Sacconi, Andrea; Pizzirusso, Dario; Salvati, Valentina; Mancini, Massimiliano; Scafetta, Giorgia; Cirombella, Roberto; Ascenzi, Francesca; Bruschini, Sara; Esposito, Antonella; Castelli, Silvia; Salvucci, Claudia; Teodonio, Leonardo; Sposato, Bruno; Catizone, Angiolina; DI NAPOLI, Arianna; Vecchione, Andrea; Ciliberto, Gennaro; Sciacchitano, Salvatore; Ricci, Alberto; Mancini, Rita. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 23:23(2022), pp. 1-12. [10.3390/ijms232315040]

B4GALT1 as a New Biomarker of Idiopathic Pulmonary Fibrosis

claudia de vitis;Dario Pizzirusso;Valentina Salvati;Giorgia Scafetta;Roberto Cirombella;Francesca Ascenzi;Sara Bruschini;Antonella Esposito;Silvia Castelli;Leonardo Teodonio;angela catizone;Arianna Di Napoli;Andrea Vecchione;Salvatore Sciacchitano
;
Alberto Ricci;Rita Mancini
2022

Abstract

Idiopathic pulmonary fibrosis (IPF) is a disease characterized by progressive scarring of the lung that involves the pulmonary interstitium. The disease may rapidly progress, leading to respiratory failure, and the long-term survival is poor. There are no accurate biomarkers available so far. Our aim was to evaluate the expression of the B4GALT1 in patients with IPF. Analysis of B4GALT1 gene expression was performed in silico on two gene sets, retrieved from the Gene Expression Omnibus database. Expression of B4GALT1 was then evaluated, both at the mRNA and protein levels, on lung specimens obtained from lung biopsies of 4 IPF patients, on one IPF-derived human primary cell and on 11 cases of IPF associated with cancer. In silico re-analysis demonstrated that the B4GALT1 gene was overexpressed in patients and human cell cultures with IPF (p = 0.03). Network analysis demonstrated that B4GALT1 upregulation was correlated with genes belonging to the EMT pathway (p = 0.01). The overexpression of B4GALT1 was observed, both at mRNA and protein levels, in lung biopsies of our four IPF patients and in the IPF-derived human primary cell, in other fibrotic non-lung tissues, and in IPF associated with cancer. In conclusion, our results indicate that B4GALT1 is overexpressed in IPF and could represent a novel marker of this disease.
2022
b4galt1; idiopathic fibrosis pulmonary; lung cancer; emt; lung fibrosis; immunohistochemistry; mrna
01 Pubblicazione su rivista::01a Articolo in rivista
B4GALT1 as a New Biomarker of Idiopathic Pulmonary Fibrosis / DE VITIS, Claudia; Michela, ; Sacconi, Andrea; Pizzirusso, Dario; Salvati, Valentina; Mancini, Massimiliano; Scafetta, Giorgia; Cirombella, Roberto; Ascenzi, Francesca; Bruschini, Sara; Esposito, Antonella; Castelli, Silvia; Salvucci, Claudia; Teodonio, Leonardo; Sposato, Bruno; Catizone, Angiolina; DI NAPOLI, Arianna; Vecchione, Andrea; Ciliberto, Gennaro; Sciacchitano, Salvatore; Ricci, Alberto; Mancini, Rita. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 23:23(2022), pp. 1-12. [10.3390/ijms232315040]
File allegati a questo prodotto
File Dimensione Formato  
DeVitis_B4GALT1-as a New-Biomarker_2022.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 2.89 MB
Formato Adobe PDF
2.89 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1673628
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 2
social impact