: The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing antibody technology (PISA), a method for selecting intrabodies against post-translationally modified proteins. We demonstrate our method by generating intrabodies against native acetylated proteins and showing functional interference in living cells.
Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies / Chirichella, Michele; Lisi, Simonetta; Fantini, Marco; Goracci, Martina; Calvello, Mariantonietta; Brandi, Rossella; Arisi, Ivan; D'Onofrio, Mara; Di Primio, Cristina; Cattaneo, Antonino. - In: NATURE METHODS. - ISSN 1548-7105. - 14:3(2017), pp. 279-282. [10.1038/nmeth.4144]
Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies
Brandi, Rossella;D'Onofrio, Mara;Cattaneo, Antonino
2017
Abstract
: The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing antibody technology (PISA), a method for selecting intrabodies against post-translationally modified proteins. We demonstrate our method by generating intrabodies against native acetylated proteins and showing functional interference in living cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
